Open Access

Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model

  • Authors:
    • Tadashi Motomura
    • Kentaro Ueda
    • Shoichiro Ohtani
    • Erlend Hansen
    • Lin Ji
    • Koichi Ito
    • Kazuyuki Saito
    • Yoichi Sugita
    • Yukihiko Nosé
  • View Affiliations

  • Published online on: September 1, 2010     https://doi.org/10.3892/or_00000896
  • Pages: 591-598
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

External microwave (EMW) hyperthermia system (2.45 GHz wave frequency) was evaluated by in vitro studies and in vivo pleural metastasis animal model. Three different non-small-cell lung cancer cells and normal fibroblast cells (control) were treated once a day for 3 days with the prototype EMW system applying mild (39°C), moderate (43°C), and severe (47°C) hyperthermia. On Day-4, tested cells were retrieved and examined by apoptosis assay kit and Western blot analysis. Cancer cells treated with moderate hyperthermia showed significant apoptosis; yet no major damage was observed to normal fibroblast cells. Western blot analysis indicated cleavage on caspase-3, -9 and PARP. Also in the cell cycle analysis, increase of sub G0-G1 population was identified. After optimization of the heating intensity for in vivo environment, we created pleural metastatic animal model in 24 immune deficiency mice (male nu/nu mice) to evaluate inhibitory effect of systemic EMW hyperthermia for disseminated tumor growth. Out of 24 mice, 8 received mild and 8 received moderate hyperthermia, and remaining 8 were the no treatment control. Whole chest area of the experimental animals was irradiated 3 times a week for 2 weeks (total of 6 time irradiations). No significant adverse event was observed including abnormal weight loss, skin burn, ulceration, and death. Metastasized tumors around the pleura and chest cavity were 75% reduced in size and weight compared to non-treated control group. Harvested tumors were stained and TUNEL assay demonstrated significant apoptosis in a moderate hyperthermia group. The EMW hyperthermia system may be possible alternative tool as a systemic hyperthermia therapy in severely advanced lung cancer patients. Further study is necessary to determine device safeness, efficacy, and synergistic effect to other possible combination therapies.

Related Articles

Journal Cover

September 2010
Volume 24 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Motomura T, Ueda K, Ohtani S, Hansen E, Ji L, Ito K, Saito K, Sugita Y and Nosé Y: Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model . Oncol Rep 24: 591-598, 2010.
APA
Motomura, T., Ueda, K., Ohtani, S., Hansen, E., Ji, L., Ito, K. ... Nosé, Y. (2010). Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model . Oncology Reports, 24, 591-598. https://doi.org/10.3892/or_00000896
MLA
Motomura, T., Ueda, K., Ohtani, S., Hansen, E., Ji, L., Ito, K., Saito, K., Sugita, Y., Nosé, Y."Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model ". Oncology Reports 24.3 (2010): 591-598.
Chicago
Motomura, T., Ueda, K., Ohtani, S., Hansen, E., Ji, L., Ito, K., Saito, K., Sugita, Y., Nosé, Y."Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model ". Oncology Reports 24, no. 3 (2010): 591-598. https://doi.org/10.3892/or_00000896