Regulation of apoptosis by p53-inducible transmembrane protein containing sushi domain

  • Authors:
    • Hongyan Cui
    • Hiroki Kamino
    • Yasuyuki Nakamura
    • Noriaki Kitamura
    • Takafumi Miyamoto
    • Daisuke Shinogi
    • Olga Goda
    • Hirofumi Arakara
    • Manabu Futamura
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/or_00000972
  • Pages:1193-1200
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Abstract

The tumor suppressor p53 is a transcription factor that induces the transcription of various target genes in response to DNA damage and it protects the cells from malignant transformation. In this study, we performed cDNA microarray analysis and found that the transmembrane protein containing sushi domain (TMPS) gene, which encodes a putative type I transmembrane protein, is a novel p53-target gene. TMPS contains a sushi domain in the extracellular region, which is associated with protein-protein interaction. TMPS expression is induced by endogenous p53 under genotoxic stress in several cancer cell lines. Reporter assay revealed p53-dependent transactivation of the p53 binding-sites (BSs) located in the intron 1 of TMPS. Chromatin immunoprecipitation (ChIP) assay showed that p53 binds to these BSs in vivo. Overexpression of TMPS induced apoptosis through the activation of caspase-3, 8, and 9 in various cancer cell lines. Moreover, γ-irradiation induced the expression of TMPS mRNA in the spleen and colon of p53+/+ mice but not in those of p53−/− mice. These data indicate that TMPS may play a role in p53-dependent apoptosis under DNA damage condition.

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November 2010
Volume 24 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
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APA
Cui, H., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, T., Shinogi, D. ... Futamura, M. (2010). Regulation of apoptosis by p53-inducible transmembrane protein containing sushi domain . Oncology Reports, 24, 1193-1200. https://doi.org/10.3892/or_00000972
MLA
Cui, H., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, T., Shinogi, D., Goda, O., Arakara, H., Futamura, M."Regulation of apoptosis by p53-inducible transmembrane protein containing sushi domain ". Oncology Reports 24.5 (2010): 1193-1200.
Chicago
Cui, H., Kamino, H., Nakamura, Y., Kitamura, N., Miyamoto, T., Shinogi, D., Goda, O., Arakara, H., Futamura, M."Regulation of apoptosis by p53-inducible transmembrane protein containing sushi domain ". Oncology Reports 24, no. 5 (2010): 1193-1200. https://doi.org/10.3892/or_00000972