Open Access

High-level expression of podoplanin in benign and malignant soft tissue tumors: Immunohistochemical and quantitative real-time RT-PCR analysis

  • Authors:
    • Yongjun Xu
    • Akira Ogose
    • Hiroyuki Kawashima
    • Tetsuo Hotta
    • Takashi Ariizumi
    • Guidong Li
    • Hajime Umezu
    • Naoto Endo
  • View Affiliations

  • Published online on: January 13, 2011     https://doi.org/10.3892/or.2011.1141
  • Pages: 599-607
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Abstract

Podoplanin is a 38 kDa mucin-type transmembrane glycoprotein that was first identified in rat glomerular epithelial cells (podocytes). It is expressed in normal lymphatic endothelium, but is absent from vascular endothelial cells. D2-40 is a commercially available mouse monoclonal antibody which binds to an epitope on human podoplanin. D2-40 immunoreactivity is therefore highly sensitive and specific for lymphatic endothelium. Recent investigations have shown widespread applications of immunohistochemical staining with D2-40 in evaluating podoplanin expression as an immunohistochemical marker for diagnosis and prognosis in various tumors. To determine whether the podoplanin (D2-40) antibody may be useful for the diagnosis of soft tissue tumors, 125 cases, including 4 kinds of benign tumors, 15 kinds of malignant tumors and 3 kinds of tumor-like lesions were immunostained using the D2-40 antibody. Total RNA was extracted from frozen tumor tissue obtained from 41 corresponding soft tissue tumor patients and 12 kinds of soft tissue tumor cell lines. Quantitative real-time PCR reactions were performed. Immunohistochemical and quantitative real-time RT-PCR analyses demonstrated the expression of the podoplanin protein and mRNA in the majority of benign and malignant soft tissue tumors and tumor-like lesions examined, with the exception of alveolar soft part sarcoma, embryonal and alveolar rhabdomyosarcoma, extraskeletal Ewing's sarcoma/peripheral primitive neuro-ectodermal tumor and lipoma, which were completely negative for podoplanin. Since it is widely and highly expressed in nearly all kinds of soft tissue tumors, especially in spindle cell sarcoma, myxoid type soft tissue tumors and soft tissue tumors of the nervous system, podoplanin is considered to have little value in the differential diagnosis of soft tissue tumors.

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March 2011
Volume 25 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xu Y, Ogose A, Kawashima H, Hotta T, Ariizumi T, Li G, Umezu H and Endo N: High-level expression of podoplanin in benign and malignant soft tissue tumors: Immunohistochemical and quantitative real-time RT-PCR analysis. Oncol Rep 25: 599-607, 2011.
APA
Xu, Y., Ogose, A., Kawashima, H., Hotta, T., Ariizumi, T., Li, G. ... Endo, N. (2011). High-level expression of podoplanin in benign and malignant soft tissue tumors: Immunohistochemical and quantitative real-time RT-PCR analysis. Oncology Reports, 25, 599-607. https://doi.org/10.3892/or.2011.1141
MLA
Xu, Y., Ogose, A., Kawashima, H., Hotta, T., Ariizumi, T., Li, G., Umezu, H., Endo, N."High-level expression of podoplanin in benign and malignant soft tissue tumors: Immunohistochemical and quantitative real-time RT-PCR analysis". Oncology Reports 25.3 (2011): 599-607.
Chicago
Xu, Y., Ogose, A., Kawashima, H., Hotta, T., Ariizumi, T., Li, G., Umezu, H., Endo, N."High-level expression of podoplanin in benign and malignant soft tissue tumors: Immunohistochemical and quantitative real-time RT-PCR analysis". Oncology Reports 25, no. 3 (2011): 599-607. https://doi.org/10.3892/or.2011.1141