Open Access

Targeting ROCK2 rather than ROCK1 inhibits Ewing sarcoma malignancy

  • Authors:
    • Rosa Simona Pinca
    • Maria Cristina Manara
    • Valentina Chiadini
    • Piero Picci
    • Cinzia Zucchini
    • Katia Scotlandi
  • View Affiliations

  • Published online on: Friday, January 20, 2017
  • Pages:1387-1393 DOI: 10.3892/or.2017.5397
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Abstract

Understanding the molecular processes characterizing Ewing sarcoma (EWS) cell migration is crucial to highlight novel therapies for patients with disseminated disease. In this study we analyzed the role of ROCK kinases in the regulation of cell migration, growth and differentiation of EWS cells. Overexpression of ROCK promotes invasion and metastasis in many solid tumors. However, the effect of ROCK in EWS has not been extensively investigated. Expression of ROCK1 and ROCK2 was analyzed by western blotting in a representative panel of human EWS cell lines, in comparison with the parameters of in vitro malignancy. We investigated the effects of a ROCK2 specific inhibitor toward those of a pan-ROCK inhibitor on the growth, migration and differentiation of two EWS cell lines. ROCK2 but not ROCK1 expression was found to be associated with in vitro cell migration and anchorage‑independent growth capabilities. Exposure of EWS cells to ROCK inhibitors significantly reduced migration and growth, while favoring morphology changes and neural differentiation. These effects were more striking when cells were specifically deprived of ROCK2 activity. Our findings lead to consider ROCK2, rather than ROCK1, as a possible molecular target for the treatment of EWS.

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Journal Cover

March 2017
Volume 37 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2015 Impact Factor: 2.486
Ranked #31/211 Oncology
(total number of cites)

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APA
Pinca, R.S., Manara, M.C., Chiadini, V., Picci, P., Zucchini, C., & Scotlandi, K. (2017). Targeting ROCK2 rather than ROCK1 inhibits Ewing sarcoma malignancy. Oncology Reports, 37, 1387-1393. http://dx.doi.org/10.3892/or.2017.5397
MLA
Pinca, R. S., Manara, M. C., Chiadini, V., Picci, P., Zucchini, C., Scotlandi, K."Targeting ROCK2 rather than ROCK1 inhibits Ewing sarcoma malignancy". Oncology Reports 37.3 (2017): 1387-1393.
Chicago
Pinca, R. S., Manara, M. C., Chiadini, V., Picci, P., Zucchini, C., Scotlandi, K."Targeting ROCK2 rather than ROCK1 inhibits Ewing sarcoma malignancy". Oncology Reports 37, no. 3 (2017): 1387-1393. http://dx.doi.org/10.3892/or.2017.5397