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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Article

Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines

  • Authors:
    • Quan-Guang Ren
    • Sheng-Li Yang
    • Jian-Li Hu
    • Pin-Dong Li
    • Ye-Shan Chen
    • Qiu-Shuang Wang
  • View Affiliations / Copyright

    Affiliations: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jianghan, Wuhan, Hubei 430022, P.R. China
  • Pages: 2270-2276
    |
    Published online on: January 13, 2016
       https://doi.org/10.3892/or.2016.4556
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Abstract

Patients with esophageal squamous cell carcinoma (ESCC) have a poor prognosis. However, the related mechanisms are unclear, thus we investigated the expression of HO-1 in ESCC tissue and explored possible mechanisms of tumor progression. Expression of HO-1 was examined by immunohistochemistry in 143 ESCC tumors. The correlation of HO-1 with clinicopathological characteristics was also examined. Two human ESCC cell lines, TE-13 and Eca109 were studied. Silencing of cell line HO-1 by specific small interfering RNA (siRNA) was evaluated using real-time quantitative PCR. Cell line viability, apoptosis and intracellular levels of reactive oxygen species (ROS) after transfection were determined using MTT and flow cytometry, respectively. HO-1, Bax, Bcl-2 and A-caspase-3/-9 expression was evaluated using western blot analyses. We found that HO-1 was expressed in 58 of 143 ESCC tumors, mainly in the cytoplasm. There was a significant association between HO-1 expression and tumor grade (P<0.001). Knockdown of HO-1 expression in cell lines was associated with significantly decreased cellular proliferation (P<0.05) and a higher rate of apoptosis (P<0.001) 48 h after treatment. Treatment of the cell lines with the ROS inhibitor N-acetylcysteine abrogated this effect. Knockdown of HO-1 was associated with increased A-caspase-3 and -9 expression, but no change in Bax or Bcl-2 expression or Bax/Bcl-2 ratio was observed. Thus, the present study identified that ESCC tumors frequently expressed HO-1. Knockdown of HO-1 promoted apoptosis through activation of a ROS-mediated caspase apoptosis pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Ren Q, Yang S, Hu J, Li P, Chen Y and Wang Q: Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines. Oncol Rep 35: 2270-2276, 2016.
APA
Ren, Q., Yang, S., Hu, J., Li, P., Chen, Y., & Wang, Q. (2016). Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines. Oncology Reports, 35, 2270-2276. https://doi.org/10.3892/or.2016.4556
MLA
Ren, Q., Yang, S., Hu, J., Li, P., Chen, Y., Wang, Q."Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines". Oncology Reports 35.4 (2016): 2270-2276.
Chicago
Ren, Q., Yang, S., Hu, J., Li, P., Chen, Y., Wang, Q."Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines". Oncology Reports 35, no. 4 (2016): 2270-2276. https://doi.org/10.3892/or.2016.4556
Copy and paste a formatted citation
x
Spandidos Publications style
Ren Q, Yang S, Hu J, Li P, Chen Y and Wang Q: Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines. Oncol Rep 35: 2270-2276, 2016.
APA
Ren, Q., Yang, S., Hu, J., Li, P., Chen, Y., & Wang, Q. (2016). Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines. Oncology Reports, 35, 2270-2276. https://doi.org/10.3892/or.2016.4556
MLA
Ren, Q., Yang, S., Hu, J., Li, P., Chen, Y., Wang, Q."Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines". Oncology Reports 35.4 (2016): 2270-2276.
Chicago
Ren, Q., Yang, S., Hu, J., Li, P., Chen, Y., Wang, Q."Evaluation of HO-1 expression, cellular ROS production, cellular proliferation and cellular apoptosis in human esophageal squamous cell carcinoma tumors and cell lines". Oncology Reports 35, no. 4 (2016): 2270-2276. https://doi.org/10.3892/or.2016.4556
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