Protective action of Citrullus colocynthis seed extracts against the deleterious effect of streptozotocin on both in vitro glucose-stimulated insulin release from rat pancreatic islets and in vivo glucose homeostasis

Benariba,Nabila; Bellakdhar,Wafaa; Djaziri,Rabeh; Hupkens,Emeline; Louchami,Karim; Malaisse,Willy  J.

doi:10.3892/br.2012.5

Protective action of Citrullus colocynthis seed extracts against the deleterious effect of streptozotocin on both in vitro glucose-stimulated insulin release from rat pancreatic islets and in vivo glucose homeostasis

Authors:
- Nabila Benariba
- Wafaa Bellakdhar
- Rabeh Djaziri
- Emeline Hupkens
- Karim Louchami
- Willy J. Malaisse
View Affiliations

Affiliations: Laboratory of Antibiotic and Antifungal Physico-Chemistry, Synthesis and Biological Activity, Faculty of Sciences, University Abou Bekr Belakaïd, Tlemcen, Algeria, Laboratory of Experimental Hormonology, Free University of Brussels, Brussels, Belgium
Published online on: August 27, 2012 https://doi.org/10.3892/br.2012.5
Pages: 119-121

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Citrullus colocynthis extracts improve glucose homeostasis in alloxan- or streptozotocin (STZ)-induced diabetic rats. Little is known, however, regarding the protective effect of these extracts against the β-cytotoxic action of STZ. In the present study, an H2O-methanol extract was found to suppress the inhibition of glucose-stimulated insulin secretion by STZ in rat-isolated pancreatic islets. Similarly, when an aqueous extract from Citrullus colocynthis seeds was injected daily for 21 days prior to STZ administration, the perturbation of glucose homeostasis otherwise generated by the β-cytotoxic agent was minimized in rats.

View Figures

View References

1.	Zaree AB, Fallahhossini F, Sharifabady R, Norooz zadeh A, Emani H and Ghoshooni H: The effect of Citrullus colocynthis extract on preventing/reducing streptozotocin-induced diabetes in rat. Kowsar Med J. 12:13–20. 2007.
2.	Dallak M, Al-Khateeb M, Al-Hashem F, Bashir N, Abbas M, Elessa R and Khalil M: In vivo, acute, normohypoglycemic, ant-hyperglycemic, insulinotropic actions of orally administered ethanol extract of Citrullus colocynthis (L.) schrab pulp. Am J Biochem Biotechnol. 5:118–125. 2009. View Article : Google Scholar
3.	Benariba N, Djaziri R, Zerriouth BH, Boucherit K, Louchami K, Sener A and Malaisse WJ: Antihyperglycemic effect of Citrullus colocynthis seed aqueous extracts in streptozotocin-induced diabetic rats. Met Funct Res Diab. 2:71–76. 2009.
4.	Malaisse-Lagae F and Malaisse WJ: Insulin release by pancreatic islets. Methods in Diabetes Research. Larner J and Pohl S: Vol I, part B.John Wiley and Sons; New York: pp. 147–152. 1984
5.	Malaisse WJ, Maggetto C, Leclercq-Meyer V and Sener A: Interference of glycogenolysis with glycolysis in pancreatic islets from glucose-infused rats. J Clin Invest. 91:432–436. 1993. View Article : Google Scholar : PubMed/NCBI
6.	Leclercq-Meyer V, Marchand J, Woussen-Colle MC, Giroix M-H and Malaisse WJ: Multiple effects of leucine on glucagon, insulin and somatostatin secretion from the perfused rat pancreas. Endocrinology. 116:1168–1174. 1985. View Article : Google Scholar : PubMed/NCBI
7.	Golden P, Baird L, Malaisse WJ, Malaisse-Lagae F and Walker M: Effect of streptozotocin on glucose-induced insulin secretion by isolated islets of Langerhans. Diabetes. 20:513–518. 1971. View Article : Google Scholar : PubMed/NCBI
8.	Golden P, Baird L, Malaisse WJ, Malaisse-Lagae F and Walker MM: Effect of 1-methyl-1-nitrosourea and streptozotocin on glucose-induced insulin secretion by isolated islets of Langerhans. Diabetologia. 12:207–209. 1976. View Article : Google Scholar : PubMed/NCBI