Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research

  • Authors:
    • Bo Tang
    • Yang Li
    • Liang Zhao
    • Shengguang Yuan
    • Zhenran Wang
    • Bo Li
    • Qian Chen
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  • Published online on: May 9, 2013     https://doi.org/10.3892/br.2013.100
  • Pages: 549-554
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Abstract

Liver cancer is a common malignant disease, with high incidence and mortality rates. The study on the proteomics of liver cancer has attracted particular attention. The quantitative study method of proteomics depends predominantly on two‑dimensional (2D) gel electrophoresis. In the present study we reported a rapid and accurate proteomics quantitative study method of high repeatability that includes the use of stable isotope labeling for the extraction of proteins and peptides via enzymolysis to achieve new type 2D capillary liquid chromatography‑mass spectrometry separation using the separation mode of cation‑exchange chromatography in conjunction with reversed‑phase chromatography. LTQ OrbiTrap mass spectrometry detection was also performed. A total of 188 differential proteins were analyzed, including 122 upregulating [deuterium/hydrogen ratio (D/H) >1.5)] and 66 downregulating proteins (D/H<0.67). These proteins may play an important role in the occurrence, drug resistance, metastasis and recurrence of cancer or other pathological processes. Such a proteomics technology may provide biological data as well as a new methodological basis for liver cancer research.
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July-August 2013
Volume 1 Issue 4

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Tang B, Li Y, Zhao L, Yuan S, Wang Z, Li B and Chen Q: Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research. Biomed Rep 1: 549-554, 2013.
APA
Tang, B., Li, Y., Zhao, L., Yuan, S., Wang, Z., Li, B., & Chen, Q. (2013). Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research. Biomedical Reports, 1, 549-554. https://doi.org/10.3892/br.2013.100
MLA
Tang, B., Li, Y., Zhao, L., Yuan, S., Wang, Z., Li, B., Chen, Q."Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research". Biomedical Reports 1.4 (2013): 549-554.
Chicago
Tang, B., Li, Y., Zhao, L., Yuan, S., Wang, Z., Li, B., Chen, Q."Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research". Biomedical Reports 1, no. 4 (2013): 549-554. https://doi.org/10.3892/br.2013.100