Suppression of metallothionein 3 gene expression by androgen in LNCaP prostate cancer cells

  • Authors:
    • Takashi Otsuka
    • Aki Hamada
    • Kazuhiro Iguchi
    • Shigeyuki Usui
    • Kazuyuki Hirano
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  • Published online on: May 17, 2013     https://doi.org/10.3892/br.2013.107
  • Pages: 614-618
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Abstract

Αndrogen deprivation therapy is the standard treatment for prostate cancer. However, tumors often progress towards a more aggressive phenotype despite treatment. Prostate tissue has a high zinc concentration, which may correlate with prostate cancer progression. Therefore, we investigated the effect of dihydrotestosterone (DHT) on the gene expression of metallothioneins (MTs) and zinc transporters in prostate cancer with quantitative real‑time polymerase chain reaction (PCR). The MT3 gene expression in LNCaP cells was suppressed by DHT in a dose‑dependent manner. However, it increased in a culture medium containing androgen‑deficient charcoal‑stripped fetal bovine serum (FBS). Bicalutamide, an androgen receptor antagonist, increased the gene expression of MT3 and partially reversed the suppression of MT3 gene expression induced by DHT. In PC‑3 cells lacking androgen receptors, DHT and bicalutamide exerted no effect on MT3 gene expression. The reporter gene assay with a luciferase reporter plasmid containing the 5'‑flanking region of MT3 demonstrated a decrease in luciferase activity caused by DHT that was reversed by bicalutamide. These results suggest that MT3 gene expression is downregulated by androgen.
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July-August 2013
Volume 1 Issue 4

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Otsuka T, Hamada A, Iguchi K, Usui S and Hirano K: Suppression of metallothionein 3 gene expression by androgen in LNCaP prostate cancer cells. Biomed Rep 1: 614-618, 2013
APA
Otsuka, T., Hamada, A., Iguchi, K., Usui, S., & Hirano, K. (2013). Suppression of metallothionein 3 gene expression by androgen in LNCaP prostate cancer cells. Biomedical Reports, 1, 614-618. https://doi.org/10.3892/br.2013.107
MLA
Otsuka, T., Hamada, A., Iguchi, K., Usui, S., Hirano, K."Suppression of metallothionein 3 gene expression by androgen in LNCaP prostate cancer cells". Biomedical Reports 1.4 (2013): 614-618.
Chicago
Otsuka, T., Hamada, A., Iguchi, K., Usui, S., Hirano, K."Suppression of metallothionein 3 gene expression by androgen in LNCaP prostate cancer cells". Biomedical Reports 1, no. 4 (2013): 614-618. https://doi.org/10.3892/br.2013.107