Preliminary study on XAGE-1b gene and its mechanism for promoting tumor cell growth

Zhou,Bo; Li,Tingxiu; Liu,Yang; Zhu,Naishuo

doi:10.3892/br.2013.122

Preliminary study on XAGE-1b gene and its mechanism for promoting tumor cell growth

Authors:
- Bo Zhou
- Tingxiu Li
- Yang Liu
- Naishuo Zhu
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Affiliations: Department of Gerontology, The Affiliated Zhongda Hospital of Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Chemotherapy, Cancer Hospital, Guangxi Medical University, Nanning, Guangxi 530000, P.R. China, Laboratory of Molecular Virology and Immunology, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China
Published online on: May 30, 2013 https://doi.org/10.3892/br.2013.122
Pages: 567-572

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Abstract

The XAGE-1b gene has been identified in numerous malignancies in the human body. However, little is known regarding its mechanism for promoting tumorigenesis in adenoid cystic carcinoma. The aim of this study was to explore the correlation between tumor cell growth and the XAGE-1b gene. The constructed PCMV‑Myc plasmid vector containing the XAGE-1b gene and transfected adenoid cystic carcinoma (ACC)‑2 cells was applied to study cell cycle alterations and anti‑apoptotic effects. These were assessed by flow cytometry with PI staining and the measurement of cell content at its Sub‑G1 phase, respectively. The fluorescence intensity representing the regulation of XAGE-1b on the transcription factors located downstream of the signaling pathway using the Mercury pathway profiling system was also detected. XAGE‑1b overexpression promoted cell growth by shortening G0-G1 and prolonging the G2-M phase. Additionally, XAGE‑1b overexpression enhanced the anti‑apoptotic effects induced by tumor necrosis factor-α (TNF-α) and serum deprivation in ACC‑2 cells. The results of the present study suggested that XAGE‑1b gene is crucial in the tumorigenesis of ACC, and its mechanism should be further investigated.

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