Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway

Koizumi,Keita; Nakajima,Hideo

doi:10.3892/br.2013.203

Serotonin induces the migration of PC12 cells via the serotonin receptor 6/cAMP/ERK pathway

Authors:
- Keita Koizumi
- Hideo Nakajima
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Affiliations: Center for Child Mental Development, Kanazawa University, Kanazawa, Ishikawa, Japan, Department of Oncology, Ageo Central General Hospital, Ageo, Saitama, Japan
Published online on: November 19, 2013 https://doi.org/10.3892/br.2013.203
Pages: 29-33

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Abstract

Serotonin (5-HT) functions as a chemoattractant that modulates neural migration during prenatal and early postnatal development. However, its molecular mechanism remains to be elucidated. The effect of 5-HT on neural cell migration was examined using PC12 neuron-like cell line. Transwell migration assay was used to determine the effect of 5-HT on PC12 cell migration. The results demonstrated that 5-HT and nerve growth factor (NGF) induced PC12 cell migration in a dose‑dependent manner. Additionally, 5-HT receptor antagonists suggest that 5-HT‑induced migration was mediated by serotonin receptor 6 (5-HT6), a Gs-protein coupled receptor that elevates the intercellular cAMP level. By contrast, antagonists of serotonin receptor 3 (5-HT3) did not show any effects on PC12 cell migration. Clozapine, an inhibitor of cAMP accumulation mediated by 5-HT6, significantly reduced the effect of 5-HT on the PC12 cell migration. An inhibitor of extracellular signal-regulated kinase (ERK) also suppressed migration. These results suggest that 5-HT induces PC12 cell migration by activating cAMP/ERK signaling pathways, which is mediated by 5-HT6 receptor.

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