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Article

Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects

  • Authors:
    • Hiroyuki Moriya
    • Katsuhiko Saito
    • Nuala Helsby
    • Naomi Hayashi
    • Shigekazu Sugino
    • Michiaki Yamakage
    • Takeru Sawaguchi
    • Masahiko Takasaki
    • Masato Takahashi
    • Nahoko Kurosawa
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Hokkaido Pharmaceutical University School of Pharmacy, Otaru, Hokkaido 047‑0264, Japan, Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, University of Auckland, Auckland 1142, New Zealand, Department of Anesthesiology, Sapporo Medical University School of Medicine, Sapporo, Hokkaido 060‑8543, Japan, Department of Pharmacy, National Hospital Organization Hokkaido Cancer Center, Sapporo, Hokkaido 003‑0804, Japan, Department of Breast Surgery, National Hospital Organization Hokkaido Cancer Center, Sapporo, Hokkaido 003‑0804, Japan
  • Pages: 265-269
    |
    Published online on: December 6, 2013
       https://doi.org/10.3892/br.2013.210
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Abstract

FcγRII and FcγRIII are low‑affinity Fcγ receptors that are encoded by the FCGR2A and FCGR3A genes, respectively. These genes contain functional single‑nucleotide polymorphisms (SNPs), which alter the binding affinities of these receptors for the γ chain of the Fc fragment of immunoglobulin G. The known SNPs in FCGR2A and FCGR3A are rs1801274 (A>G; H131R) and rs396991 (T>G; F158V), respectively. It is also known that there are copy number variations (CNVs) in the genetic locus (1q23) where FCGR2A and FCGR3A are located. However, the frequencies of these SNPs and CNVs have not been determined in the Japanese population. The aim of this study was to investigate SNPs and CNVs in FCGR2A and FCGR3A among 113 healthy individuals. The SNPs and CNVs in FCGR2A and FCGR3A were determined using the TaqMan® SNP Genotyping and the TaqMan® Copy Number assays. Our results revealed that the incidence of FCGR2A (rs1801274) genotypes were as follows: A̸A, 69.9%; A̸G, 29.2%; and G̸G, 0.9%. The incidence of the FCGR3A (rs396991) genotypes were as follows: T̸T, 56.7%; T̸G, 38.9%; and G̸G, 4.4%). No CNVs were detected for FCGR2A. To the best of our knowledge, this finding has not been previously reported in the Japanese population. By contrast, CNVs were observed in FCGR3A (3 subjects were found to harbour a gene deletion and 5 subjects had 3 copies of the gene). Using simple commercially available assays we were able to confirm previous findings regarding FCGR2A and FCGR3A alleles and CNVs. These assays may provide a basis for the investigation of the role of these genes in the efficacy of antibody‑based drugs, such as trastuzumab and rituximab, in Japanese subjects.
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Copy and paste a formatted citation
Spandidos Publications style
Moriya H, Saito K, Helsby N, Hayashi N, Sugino S, Yamakage M, Sawaguchi T, Takasaki M, Takahashi M, Kurosawa N, Kurosawa N, et al: Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects. Biomed Rep 2: 265-269, 2014.
APA
Moriya, H., Saito, K., Helsby, N., Hayashi, N., Sugino, S., Yamakage, M. ... Kurosawa, N. (2014). Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects. Biomedical Reports, 2, 265-269. https://doi.org/10.3892/br.2013.210
MLA
Moriya, H., Saito, K., Helsby, N., Hayashi, N., Sugino, S., Yamakage, M., Sawaguchi, T., Takasaki, M., Takahashi, M., Kurosawa, N."Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects". Biomedical Reports 2.2 (2014): 265-269.
Chicago
Moriya, H., Saito, K., Helsby, N., Hayashi, N., Sugino, S., Yamakage, M., Sawaguchi, T., Takasaki, M., Takahashi, M., Kurosawa, N."Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects". Biomedical Reports 2, no. 2 (2014): 265-269. https://doi.org/10.3892/br.2013.210
Copy and paste a formatted citation
x
Spandidos Publications style
Moriya H, Saito K, Helsby N, Hayashi N, Sugino S, Yamakage M, Sawaguchi T, Takasaki M, Takahashi M, Kurosawa N, Kurosawa N, et al: Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects. Biomed Rep 2: 265-269, 2014.
APA
Moriya, H., Saito, K., Helsby, N., Hayashi, N., Sugino, S., Yamakage, M. ... Kurosawa, N. (2014). Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects. Biomedical Reports, 2, 265-269. https://doi.org/10.3892/br.2013.210
MLA
Moriya, H., Saito, K., Helsby, N., Hayashi, N., Sugino, S., Yamakage, M., Sawaguchi, T., Takasaki, M., Takahashi, M., Kurosawa, N."Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects". Biomedical Reports 2.2 (2014): 265-269.
Chicago
Moriya, H., Saito, K., Helsby, N., Hayashi, N., Sugino, S., Yamakage, M., Sawaguchi, T., Takasaki, M., Takahashi, M., Kurosawa, N."Single‑nucleotide polymorphisms and copy number variations of the FCGR2A and FCGR3A genes in healthy Japanese subjects". Biomedical Reports 2, no. 2 (2014): 265-269. https://doi.org/10.3892/br.2013.210
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