Association of ‑108 C>T PON1 polymorphism with polycystic ovary syndrome

Ferk,Polonca; Gersak,Ksenija

doi:10.3892/br.2013.214

Association of ‑108 C>T PON1 polymorphism with polycystic ovary syndrome

Authors:
- Polonca Ferk
- Ksenija Gersak
View Affiliations

Affiliations: Department of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Maribor, Maribor, Slovenia, Department of Obstetrics and Gynaecology, Ljubljana University Medical Centre, Ljubljana, Slovenia
Published online on: December 30, 2013 https://doi.org/10.3892/br.2013.214
Pages: 255-259

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Polycystic ovary syndrome (PCOS) is possibly the most common endocrine disorder in premenopausal women. in this study, we aimed to investigate the association of the ‑108 C>T polymorphism in the PON1 gene, which encodes the antioxidant enzyme paraoxonase‑1, with PCOS. A total of 118 women with PCOS and 108 control subjects were included in this case‑control study. the PON1 polymorphism was genotyped, biochemical and clinical parameters were determined and the correlations between the parameters were statistically evaluated. The differences in the PON1 allele and genotype distributions between PCOS patients and controls did not reach a statistical significance. The serum fasting glucose (GLU) levels did not differ significantly between the PCOS patients and the controls. However, the serum fasting insulin (INS) concentration, INS̸GLU ratio and homeostasis model assessment (HOMA) index, although within the normal range, were significantly higher in the PCOS group. When considering PCOS patients and controls as separate groups or as a single group of patients, none of the analyzed biochemical or clinical parameters were found to be significantly correlated with the PON1 polymorphism. Therefore, the ‑108 C>T PON1 polymorphism was not found to be significantly associated with the presence of PCOS or with its particular clinical and biochemical characteristics in non‑insulin resistant, non‑obese patients.

View Figures

View References

1	Homburg R: What is polycystic ovarian syndrome? A proposal for a consensus on the definition and diagnosis of polycystic ovarian syndrome. Hum Reprod. 17:2495–2499. 2002. View Article : Google Scholar : PubMed/NCBI
2	Homburg R: The management of infertility associated with polycystic ovary syndrome. Reprod Biol Endocrinol. 1:1092003. View Article : Google Scholar : PubMed/NCBI
3	Diamanti-Kandarakis E, Piperi C, Spina J, Argyrakopoulou G, Papanastasiou L, Bergiele A and Panidis D: Polycystic ovary syndrome: the influence of environmental and genetic factors. Hormones (Athens). 5:17–34. 2006. View Article : Google Scholar
4	Mukherjee S and Maitra A: Molecular and genetic factors contributing to insulin resistance in polycystic ovary syndrome. Indian J Med Res. 131:743–760. 2010.PubMed/NCBI
5	Prapas N, Karkanaki A, Prapas I, Kalogiannidis I, Katsikis I and Panidis D: Genetics of polycystic ovary syndrome. Hippokratia. 13:216–223. 2009.PubMed/NCBI
6	Lenarcik A, Bidzinska-Speichert B and Tworowska-Bardzinska U: The role of chronic inflammation and Leu55Met PON1 polymorphism in the pathogenesis of polycystic ovary syndrome. Gynecol Endocrinol. 26:673–683. 2010. View Article : Google Scholar : PubMed/NCBI
7	Luque-Ramirez M, San Millan JL and Escobar-Morreale HF: Genomic variants in polycystic ovary syndrome. Clin Chim Acta. 366:14–26. 2006. View Article : Google Scholar : PubMed/NCBI
8	Martinez-Garcia MA, Luque-Ramirez M, San-Millan JL and Escobar-Morreale HF: Body iron stores and glucose intolerance in premenopausal women: role of hyperandrogenism, insulin resistance, and genomic variants related to inflammation, oxidative stress, and iron metabolism. Diabetes Care. 32:1525–1530. 2009. View Article : Google Scholar
9	San Millan JL, Alvarez-Blasco F, Luque-Ramirez M, Botella-Carretero JI and Escobar-Morreale HF: The PON1-108C/T polymorphism, and not the polycystic ovary syndrome, is an important determinant of reduced serum paraoxonase activity in premenopausal women. Hum Reprod. 21:3157–3161. 2006.PubMed/NCBI
10	Mohamadin AM, Habib FA and Elahi TF: Serum paraoxonase 1 activity and oxidant/antioxidant status in Saudi women with polycystic ovary syndrome. Pathophysiology. 17:189–196. 2010. View Article : Google Scholar : PubMed/NCBI
11	Fenkci IV, Serteser M, Fenkci S and Kose S: Paraoxonase levels in women with polycystic ovary syndrome. J Reprod Med. 52:879–883. 2007.PubMed/NCBI
12	Dursun P, Demirtas E, Bayrak A and Yarali H: Decreased serum paraoxonase 1 (PON1) activity: an additional risk factor for atherosclerotic heart disease in patients with PCOS? Hum Reprod. 21:104–108. 2006. View Article : Google Scholar : PubMed/NCBI
13	San Millan JL, Corton M, Villuendas G, Sancho J, Peral B and Escobar-Morreale HF: Association of the polycystic ovary syndrome with genomic variants related to insulin resistance, type 2 diabetes mellitus, and obesity. J Clin Endocrinol Metab. 89:2640–2646. 2004.PubMed/NCBI
14	Brophy VH, Jampsa RL, Clendenning JB, McKinstry LA, Jarvik GP and Furlong CE: Effects of 5′ regulatory-region polymorphisms on paraoxonase-gene (PON1) expression. Am J Hum Genet. 68:1428–1436. 2001.
15	Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril. 81:19–25. 2004. View Article : Google Scholar
16	Balen AH, Laven JS, Tan SL and Dewailly D: Ultrasound assessment of the polycystic ovary: international consensus definitions. Hum Reprod Update. 9:505–514. 2003. View Article : Google Scholar : PubMed/NCBI
17	Ly LP and Handelsman DJ: Empirical estimation of free testosterone from testosterone and sex hormone-binding globulin immunoassays. Eur J Endocrinol. 152:471–478. 2005. View Article : Google Scholar : PubMed/NCBI
18	Ferk P, Perme MP and Gersak K: Insulin gene polymorphism in women with polycystic ovary syndrome. J Int Med Res. 36:1180–1187. 2008. View Article : Google Scholar : PubMed/NCBI
19	Soyman Z, Noyan V, Tulmac M, Yucel A, Sagsoz N, Bayrak T and Cakir E: Serum paraoxonase 1 activity, asymmetric dimethylarginine levels, and brachial artery flow-mediated dilatation in women with polycystic ovary syndrome. Fertil Steril. 95:1067–1072. 2011. View Article : Google Scholar
20	Leviev I, Kalix B, Brulhart Meynet MC and James RW: The paraoxonase PON1 promoter polymorphism C(-107)T is associated with increased serum glucose concentrations in non-diabetic patients. Diabetologia. 44:1177–1183. 2001. View Article : Google Scholar : PubMed/NCBI
21	Zhang F, Liu HW, Fan P, Bai H and Song Q: The -108 C/T polymorphism in paraoxonase 1 gene in Chinese patients with polycystic ovary syndrome. J Sichuan Univ Med Sci Ed. 42:24–28. 2011.(In Chinese).
22	Torun AN, Vural M, Cece H, Camuzcuoglu H, Toy H and Aksoy N: Paraoxonase-1 is not affected in polycystic ovary syndrome without metabolic syndrome and insulin resistance, but oxidative stress is altered. Gynecol Endocrinol. 27:988–992. 2011. View Article : Google Scholar : PubMed/NCBI
23	Hughes C, Elgasim M, Layfield R and Atiomo W: Genomic and post-genomic approaches to polycystic ovary syndrome - progress so far: Mini Review. Hum Reprod. 21:2766–2775. 2006. View Article : Google Scholar : PubMed/NCBI
24	Simoni M, Tempfer CB, Destenaves B and Fauser BC: Functional genetic polymorphisms and female reproductive disorders: Part I: Polycystic ovary syndrome and ovarian response. Hum Reprod Update. 14:459–484. 2008. View Article : Google Scholar : PubMed/NCBI
25	Lander ES and Schork NJ: Genetic dissection of complex traits. Science. 265:2037–2048. 1994. View Article : Google Scholar : PubMed/NCBI
26	Unluturk U, Harmanci A, Kocaefe C and Yildiz BO: The genetic basis of the polycystic ovary syndrome: a literature review including discussion of PPAR-gamma. PPAR Res. 2007:491092007. View Article : Google Scholar : PubMed/NCBI
27	Skerget AE, Herodez SS, Zagorac A, Zagradisnik B and Vokac NK: The use of comparative genomic hybridization as a diagnostic tool in medical genetics laboratories: first application on different clinical samples. Acta Medico-Biotechnica. 4:49–56. 2011.
28	Goodarzi MO: Looking for polycystic ovary syndrome genes: rational and best strategy. Semin Reprod Med. 26:5–13. 2008. View Article : Google Scholar : PubMed/NCBI
29	Pasquali R, Stener-Victorin E, Yildiz BO, et al: PCOS Forum: research in polycystic ovary syndrome today and tomorrow. Clin Endocrinol (Oxf). 74:424–433. 2011. View Article : Google Scholar : PubMed/NCBI