Association of Toll‑like receptor 2 polymorphisms with gout

Cai,Yan; Peng,Yi‑Hua; Tang,Zhong; Guo,Xiao‑Lan; Qing,Yu‑Feng; Liang,Su‑Hua; Jiang,Hong; Dang,Wang‑Tai; Ma,Qiang; He,Cheng; Zhou,Jing‑Guo

doi:10.3892/br.2014.224

Association of Toll‑like receptor 2 polymorphisms with gout

Authors:
- Yan Cai
- Yi‑Hua Peng
- Zhong Tang
- Xiao‑Lan Guo
- Yu‑Feng Qing
- Su‑Hua Liang
- Hong Jiang
- Wang‑Tai Dang
- Qiang Ma
- Cheng He
- Jing‑Guo Zhou
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Affiliations: Faculty of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Endocrinology, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Institute of Rheumatism Immunity, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China , Department of Biology, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China
Published online on: January 17, 2014 https://doi.org/10.3892/br.2014.224
Pages: 292-296

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Abstract

Gout is the most common autoinflammatory arthritis characterized by elevated serum urate and recurrent attacks of intra‑articular crystal deposition of monosodium urate (MSU) in tissues. The pathogenesis of gout has not been fully determined, although certain genetic factors are involved in the development of gout. Accumulated data suggested that MSU crystal‑induced inflammation is a paradigm of innate immunity. As Toll‑like receptors (TLRs) are the underlying mechanisms of the innate immune response, the present study aimed to investigate whether TLR2 polymorphisms are associated with gout. Two single‑nucleotide polymorphisms (Arg677Trp and Arg753Gln, rs5743708) in TLR2 were genotyped by polymerase chain reaction‑restriction fragment length polymorphism and the ‑196 to ‑174 del polymorphism was investigated using the allele‑specific polymerase chain reaction in 431 individuals (215 patients with gout and 216 healthy controls). TLR2 Arg677Trp and Arg753Gln genotyping indicated that all the positive samples were of the wild‑type genotype. No significant differences in genotype (χ2=1.686, P=0.430) and allele (χ2=1.430, P=0.232) frequencies of the ‑196 to ‑174 del polymorphism between the patients with gout and the control groups was observed. Our results suggested that the TLR2 Arg677Trp, Arg753Gln and the ‑196 to ‑174 del polymorphisms were not associated with susceptibility to primary gouty arthritis.

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