Plasma microRNA‑320, microRNA‑let‑7e and microRNA‑21 as novel potential biomarkers for the detection of retinoblastoma

  • Authors:
    • Shan‑Shan Liu
    • Yu‑Shi Wang
    • Yan‑Feng Sun
    • Li‑Xia Miao
    • Jun Wang
    • Yan‑Shan Li
    • Hong‑Yan Liu
    • Qiu‑Ling Liu
  • View Affiliations

  • Published online on: March 7, 2014     https://doi.org/10.3892/br.2014.246
  • Pages: 424-428
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Abstract

Retinoblastoma (RB) is a childhood malignancy caused by inactivation of the RB gene, with neuron‑specific enolase (NSE) levels considered as its diagnostic marker. microRNAs (miRNAs) have been proven to play a significant role in multiple physiological and pathological processes and several miRNAs were identified as tumor biomarkers in recent studies. In the present study, 65 plasma samples were collected from RB patients and 65 samples from healthy individuals to serve as controls. The miRNA levels were measured via quantitative reverse transcription-polymerase chain reaction and their association with RB was assessed by statistical data analysis and receiver operating characteristic curves. Plasma miRNA (miR)‑320, miR‑let‑7e and miR‑21 levels were downregulated in the patient samples, the areas under the curves (AUCs) were 0.548‑0.660, whereas the AUCs of combined classifiers were ≥0.990. The plasma miRNA levels, particularly of miR‑320, were found to be of value in RB diagnosis and may be considered as novel diagnostic biomarkers.
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May-June 2014
Volume 2 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Liu SS, Wang YS, Sun YF, Miao LX, Wang J, Li YS, Liu HY and Liu QL: Plasma microRNA‑320, microRNA‑let‑7e and microRNA‑21 as novel potential biomarkers for the detection of retinoblastoma. Biomed Rep 2: 424-428, 2014
APA
Liu, S., Wang, Y., Sun, Y., Miao, L., Wang, J., Li, Y. ... Liu, Q. (2014). Plasma microRNA‑320, microRNA‑let‑7e and microRNA‑21 as novel potential biomarkers for the detection of retinoblastoma. Biomedical Reports, 2, 424-428. https://doi.org/10.3892/br.2014.246
MLA
Liu, S., Wang, Y., Sun, Y., Miao, L., Wang, J., Li, Y., Liu, H., Liu, Q."Plasma microRNA‑320, microRNA‑let‑7e and microRNA‑21 as novel potential biomarkers for the detection of retinoblastoma". Biomedical Reports 2.3 (2014): 424-428.
Chicago
Liu, S., Wang, Y., Sun, Y., Miao, L., Wang, J., Li, Y., Liu, H., Liu, Q."Plasma microRNA‑320, microRNA‑let‑7e and microRNA‑21 as novel potential biomarkers for the detection of retinoblastoma". Biomedical Reports 2, no. 3 (2014): 424-428. https://doi.org/10.3892/br.2014.246