Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells

Cetik,Sibel; Rzajeva,Aigun; Malaisse,Willy  J.; Sener,Abdullah

doi:10.3892/br.2014.269

Effect of cytochalasin B on 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose handling by BRIN‑BD11 cells

Authors:
- Sibel Cetik
- Aigun Rzajeva
- Willy J. Malaisse
- Abdullah Sener
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Affiliations: Laboratory of Experimental Hormonology, Brussels Free University, Brussels B‑1070, Belgium
Published online on: April 25, 2014 https://doi.org/10.3892/br.2014.269
Pages: 513-516

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Abstract

The present study aimed to investigate the effects of cytochalasin B (20 µM) on the uptake of 3‑O‑[14C]‑methyl‑D‑glucose or D‑[U‑14C]glucose (8.3 mM each) by BRIN‑BD11 cells. Taking into account the distribution space of tritiated water (3HOH), which was unexpectedly increased shortly after exposure of the cells to cytochalasin B and then progressively returned to its control values, and that of L‑[1‑14C]glucose, used as an extracellular marker, it was demonstrated that cytochalasin B caused a modest, but significant inhibition of the uptake of D‑glucose and its non‑metabolized analog by the BRIN‑BD11 cells. These findings resemble those observed in acinar or ductal cells of the rat submaxillary gland and displayed a relative magnitude comparable to that found for the inhibition of D‑glucose metabolism by cytochalasin B in purified pancreatic islet B cells. These findings reinforce the view that the primary site of action of cytochalasin B is located at the level of the plasma membrane.

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