Effect of pertussis toxin on calcium influx in three contraction models

Grześk,Elżbieta; Tejza,Barbara; Wiciński,Michał; Malinowski,Bartosz; Szadujkis‑Szadurska,Katarzyna; Baran,Lilianna; Kowal,Elżbieta; Grześk,Grzegorz

doi:10.3892/br.2014.274

Effect of pertussis toxin on calcium influx in three contraction models

Authors:
- Elżbieta Grześk
- Barbara Tejza
- Michał Wiciński
- Bartosz Malinowski
- Katarzyna Szadujkis‑Szadurska
- Lilianna Baran
- Elżbieta Kowal
- Grzegorz Grześk
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Affiliations: Department of Pediatrics, Hematology and Oncology, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz 85094, Poland, Department of Pharmacology and Therapeutics, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz 85094, Poland
Published online on: May 12, 2014 https://doi.org/10.3892/br.2014.274
Pages: 584-588

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Abstract

Pertussis toxin (PTX) blocks G protein activation and inhibits signal transmission from the activated receptor to effectors that are specific for the G protein‑coupled receptor. The aim of the present study was to evaluate the effect of PTX on vascular smooth muscle cells that were stimulated pharmacologically with phenylephrine (α-adrenoceptor agonist), mastoparan‑7 (direct G‑protein activator) and Bay K8644 (direct calcium channel activator). The changes in perfusion pressure that were proportional to the degree of phenylephrine‑induced constriction of rat tail arteries were assessed. Concentration‑response curves (CRCs) that were obtained for phenylephrine, mastoparan‑7 and Bay K8644 presented a sigmoidal association. A significantly reduced calcium influx to the cytoplasm in the presence of mastoparan‑7 resulted in a significant rightward shift of the CRCs with a significant reduction in maximal responses. The presence of PTX did not change mastoparan‑7 and Bay K8644‑induced contraction, whereas the significant inhibition of phenylephrine‑induced contraction was found. The results of the experiments indicated that PTX significantly inhibited phenylephrine‑induced contraction of vascular smooth muscle cells by inhibition of calcium influx from the intra‑ and extracellular calcium space. PTX did not change the smooth muscle contraction that was induced by mastoparan‑7 and Bay K8644. The predominant effect of mastoparan‑7 may be associated with other binding sites as compared to the G‑protein or PTX may bind to other sites than mastoparan‑7.

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