Effects of ionizing radiation on retinoic acid-inducible gene-I-like receptors
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- Published online on: November 7, 2014 https://doi.org/10.3892/br.2014.377
- Pages: 59-62
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Abstract
Retinoic acid‑inducible gene‑I (RIG‑I)‑like receptors [RLRs; RIG‑I and melanoma differentiation‑associated gene 5 (MDA5)] sense virus‑derived RNA or a synthetic analog of double‑stranded RNA polyinosinic‑polycytidylic acid [poly(I:C)] and are responsible for host defense against viruses. However, it remains unclear whether radiation affects RLRs. Therefore, the present study investigated the effects of ionizing radiation on RIG‑I and MDA5 expression and the response to poly(I:C) using THP1 (human monocytic cell line)‑derived macrophages. Non‑ and X‑irradiated (1‑10 Gy) macrophages expressed RIG‑I and MDA5 at mRNA and protein levels and there was no significant difference in the expression levels. Non‑ and X‑irradiated macrophages expressed antiviral cytokine interferon (IFN)‑β mRNA following poly(I:C)‑low molecular weight/LyoVec™ and poly(I:C)‑high molecular weight/LyoVec™ stimulation, the agonist of RIG‑I and MDA5, respectively. In line with the results of the expression of RIG‑I and MDA5, no significant difference in the expression of IFN‑β mRNA was observed between non‑ and X‑irradiation. These results indicate that ionizing radiation hardly affects RLR expression and the response to their agonist poly(I:C) in THP1‑derived macrophages.
