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Article

Isolation and genetic characterization of enterovirus in patients with febrile rash illness

  • Authors:
    • Zhaohui Ni
    • Fengyang Xiang
    • Honglan Huang
    • Guoqing Wang
    • Fan Li
  • View Affiliations / Copyright

    Affiliations: Department of Pathogenobiology, The Key Laboratory of Zoonosis, Chinese Ministry of Education, College of Basic Medical Science, Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 375-378
    |
    Published online on: March 5, 2015
       https://doi.org/10.3892/br.2015.441
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Abstract

Measles and rubella virus are usually considered as the causative agents in patients with febrile rash illness (FRI). However, investigators have identified that enteroviruses are also associated with FRI, and the present study was undertaken to investigate this association. In the study, 20 throat swab samples were collected from patients suffering from rash and fever between April and July in 2013. The 20 samples tested negative for measles virus, but all were positive when reverse transcription‑polymerase chain reaction was performed using enterovirus universal primers. Specifically, five tested positive for Coxsackie B3 virus (CVB3). The titers of the five CVB3 isolates were 106.5, 106.4, 107.5, 106.5 and 106.5 tissue culture infectious dose50/100 µl respectively. The partial VP1 sequences of the five CVB3 isolates were identical to each other and were closely associated with the CVB3/MKP and CVB3/Macocy strains, which are known to induce myocarditis and neonatal diseases of the central nervous system. In conclusion, CVB3 may cause symptoms of fever and rash and should be differentiated from measles, rubella and other infectious pathogens. The five CVB3 isolates described in the study were genetically similar to each other and to other local CVB3 strains. The results provide further data on the viral pathogen spectrum associated with FRI.
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Copy and paste a formatted citation
Spandidos Publications style
Ni Z, Xiang F, Huang H, Wang G and Li F: Isolation and genetic characterization of enterovirus in patients with febrile rash illness. Biomed Rep 3: 375-378, 2015.
APA
Ni, Z., Xiang, F., Huang, H., Wang, G., & Li, F. (2015). Isolation and genetic characterization of enterovirus in patients with febrile rash illness. Biomedical Reports, 3, 375-378. https://doi.org/10.3892/br.2015.441
MLA
Ni, Z., Xiang, F., Huang, H., Wang, G., Li, F."Isolation and genetic characterization of enterovirus in patients with febrile rash illness". Biomedical Reports 3.3 (2015): 375-378.
Chicago
Ni, Z., Xiang, F., Huang, H., Wang, G., Li, F."Isolation and genetic characterization of enterovirus in patients with febrile rash illness". Biomedical Reports 3, no. 3 (2015): 375-378. https://doi.org/10.3892/br.2015.441
Copy and paste a formatted citation
x
Spandidos Publications style
Ni Z, Xiang F, Huang H, Wang G and Li F: Isolation and genetic characterization of enterovirus in patients with febrile rash illness. Biomed Rep 3: 375-378, 2015.
APA
Ni, Z., Xiang, F., Huang, H., Wang, G., & Li, F. (2015). Isolation and genetic characterization of enterovirus in patients with febrile rash illness. Biomedical Reports, 3, 375-378. https://doi.org/10.3892/br.2015.441
MLA
Ni, Z., Xiang, F., Huang, H., Wang, G., Li, F."Isolation and genetic characterization of enterovirus in patients with febrile rash illness". Biomedical Reports 3.3 (2015): 375-378.
Chicago
Ni, Z., Xiang, F., Huang, H., Wang, G., Li, F."Isolation and genetic characterization of enterovirus in patients with febrile rash illness". Biomedical Reports 3, no. 3 (2015): 375-378. https://doi.org/10.3892/br.2015.441
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