Advanced glycation end products upregulate angiopoietin-like protein 4 expression by activating the renin-angiotensin system in endothelial cells

Zheng,Zhenda; Cheng,Cailian; Dong,Ruimin; Li,Mei; Xiong,Zhaojun; Qian,Xiaoxian; Chen,Lin

doi:10.3892/br.2015.468

Advanced glycation end products upregulate angiopoietin-like protein 4 expression by activating the renin-angiotensin system in endothelial cells

Authors:
- Zhenda Zheng
- Cailian Cheng
- Ruimin Dong
- Mei Li
- Zhaojun Xiong
- Xiaoxian Qian
- Lin Chen
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Affiliations: Department of Cardiovascular, The Third Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China, Department of Nephrology, The Third Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China
Published online on: May 21, 2015 https://doi.org/10.3892/br.2015.468
Pages: 578-582

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Abstract

The aim of the present study was to investigate the effects of advanced glycation end products (AGEs) on the expression of angiopoietin-like protein 4 (ANGPTL4) and the mechanisms of the effects in endothelial cells. Endothelial cells were incubated with various concentrations of AGEs for 24 h and the expression of ANGPTL4 was detected by quantitative polymerase chain reaction and western blot analysis. The concentration of angiotensin Ⅱ (Ang Ⅱ) in conditioned media and cell lysates was measured by enzyme‑linked immunosorbent assays. Fluorescein isothiocyanate‑labeled dextran filtration assays and transendothelial electrical resistance were performed to evaluate endothelial permeability. AGEs (80 µg/ml) increased the expression of ANGPTL4 and the levels of Ang Ⅱ (P<0.05). Incubation with AGEs also resulted in a significant increase in endothelial permeability (P<0.05). However, pretreatment with the Ang Ⅱ receptor blocker losartan (10‑5 M) reduced the effects of AGEs (P<0.05). AGEs upregulated the expression of ANGPTL4 by activating a local renin‑angiotensin system in endothelial cells. This may be a new mechanism by which AGEs increase endothelial permeability.

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