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V‑PYRRO/NO downregulates mRNA expression levels of leukotriene C4 synthase during hepatic ischemia reperfusion injury in rats via inhibition of the nuclear factor‑κB activation pathway

  • Authors:
    • Feng‑Fang Hong
    • Yi‑Fan Wang
    • Hui Liu
    • Mei‑Wen Yang
    • Shu‑Long Yang

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  • Published online on: October 16, 2015     https://doi.org/10.3892/br.2015.533
  • Pages: 112-116
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Abstract

The aim of the present study was to explore the mechanism underlying the effects of a selective liver nitric oxide (NO) donor, O2-vinyl1-(pyrrolidin-1-yl)-diazen-1-ium-1,2-diolate (V-PYRRO/NO), on the gene expression of leukotriene C4 synthase (LTC4S) during hepatic ischemia/reperfusion (I/R). Adult male Sprague-Dawley rats were divided into 3 groups: Sham (control), I/R and V-PYRRO/NO + I/R groups. The liver was subjected to 1 h of partial hepatic ischemia followed by 5 h of reperfusion, saline or V-PYRRO/NO (1.06 µmol/kg/h) administered intravenously. The mRNA expression levels of LTC4S in rat liver tissue were examined by the reverse transcription-polymerase chain reaction method, the protein expression levels of nuclear factor-κB (NF-κB) p65, p50 and IκBα in liver cell lysates and nuclear extracts were detected by western blot analysis. Hepatic mRNA expression of LTC4S was lower in V-PYRRO/NO + I/R group compared to the I/R group. In addition, the protein expression levels of NF-κB p65 and p50 in the nucleus extract were lower in the V-PYRRO/NO + I/R group when compared with the I/R group. However, the IκBα protein in the 3 groups was not changed. Immunohistochemistry staining revealed that the I/R liver exhibited strong cytoplasmic and nuclear staining for NF-κB p65; however, the V-PYRRO/NO + I/R group liver presented slight cytoplasmic and nuclear staining. In conclusion, V-PYRRO/NO may downregulate LTC4S mRNA expression by inhibiting NF-κB activation independent of IκBα during hepatic I/R injury.
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January-2016
Volume 4 Issue 1

Print ISSN: 2049-9434
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Spandidos Publications style
Hong FF, Wang YF, Liu H, Yang MW and Yang SL: V‑PYRRO/NO downregulates mRNA expression levels of leukotriene C4 synthase during hepatic ischemia reperfusion injury in rats via inhibition of the nuclear factor‑κB activation pathway. Biomed Rep 4: 112-116, 2016.
APA
Hong, F., Wang, Y., Liu, H., Yang, M., & Yang, S. (2016). V‑PYRRO/NO downregulates mRNA expression levels of leukotriene C4 synthase during hepatic ischemia reperfusion injury in rats via inhibition of the nuclear factor‑κB activation pathway. Biomedical Reports, 4, 112-116. https://doi.org/10.3892/br.2015.533
MLA
Hong, F., Wang, Y., Liu, H., Yang, M., Yang, S."V‑PYRRO/NO downregulates mRNA expression levels of leukotriene C4 synthase during hepatic ischemia reperfusion injury in rats via inhibition of the nuclear factor‑κB activation pathway". Biomedical Reports 4.1 (2016): 112-116.
Chicago
Hong, F., Wang, Y., Liu, H., Yang, M., Yang, S."V‑PYRRO/NO downregulates mRNA expression levels of leukotriene C4 synthase during hepatic ischemia reperfusion injury in rats via inhibition of the nuclear factor‑κB activation pathway". Biomedical Reports 4, no. 1 (2016): 112-116. https://doi.org/10.3892/br.2015.533