Assessment of biochemical parameters and characterization of TNFα -308G/A and PTPN22 +1858C/T gene polymorphisms 
in the risk of obesity in adolescents

Salinas‑Santander,Mauricio   Andrés; León‑Cachón,Rafael  Baltazar; Cepeda‑Nieto,Ana  Cecilia; Sánchez‑Domínguez,Celia  Nohemí; González-Zavala,María  Antonia; Gallardo‑Blanco,Hugo  Leonid; Esparza‑González,Sandra  Cecilia; González-Madrazo,Miguel  Ángel

doi:10.3892/br.2015.534

Assessment of biochemical parameters and characterization of TNFα -308G/A and PTPN22 +1858C/T gene polymorphisms in the risk of obesity in adolescents

Authors:
- Mauricio Andrés Salinas‑Santander
- Rafael Baltazar León‑Cachón
- Ana Cecilia Cepeda‑Nieto
- Celia Nohemí Sánchez‑Domínguez
- María Antonia González-Zavala
- Hugo Leonid Gallardo‑Blanco
- Sandra Cecilia Esparza‑González
- Miguel Ángel González-Madrazo
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Affiliations: Research Department, Saltillo Unit Faculty of Medicine, Autonomous University of Coahuila, Saltillo, Coahuila CP 2500, Mexico, Department of Basic Sciences, Division of Health Sciences, University of Monterrey, San Pedro Garza García, Nuevo León CP 66238, Mexico, Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Autonomous University of Nuevo León, Monterrey, Nuevo León CP 64460, Mexico, School of Chemical Sciences, Autonomous University of Coahuila, Saltillo, Coahuila CP 25280, Mexico, Department of Genetics, School of Medicine, Autonomous University of Nuevo León, Monterrey, Nuevo León CP 64460, Mexico
Published online on: October 27, 2015 https://doi.org/10.3892/br.2015.534
Pages: 107-111

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Abstract

Obesity is currently considered an inflammatory condition associated with autoimmune diseases, suggesting a common origin. Among other factors, candidate genes may explain the development of this disease. Polymorphisms in the tumor necrosis factor α (TNFα) and lymphoid protein tyrosine phosphatase (PTPN22) genes lead to an increased risk to development of immune and inflammatory diseases. The aim of the present study was to analyze the biochemical parameters and the effect of the TNFα ‑308G/A and PTPN22 +1858C/T polymorphisms in the susceptibility of adolescents to obesity. A group of 253 adolescent subjects were recruited and classified as obese, overweight or normal weight according to their nutritional status. Anthropometric measurements, clinical and biochemical data were analyzed. DNA was extracted from peripheral blood samples by the phenol‑chloroform method, and TNFα ‑308G/A and PTPN22 1858C/T polymorphisms were determined by polymerase chain reaction‑restriction fragment length polymorphism assays. Clinical, genetic and biochemical parameters were analyzed to determine the existence of a possible association with the development of obesity. Statistically significant differences in body mass index, insulin, triglyceride levels and homeostatic model assessment for insulin resistance (HOMA‑IR) index were observed among the three groups analyzed (P≤0.05). The studied polymorphisms did not confer a risk for developing obesity in the analyzed population (P>0.05); however, significantly low levels of insulin and decreased rates of HOMA‑IR were observed in the 1858 CT genotype carriers of the PTPN22 gene. In conclusion, no association between the TNFα ‑308G/A and PTPN22 +1858C/T polymorphisms and the risk to development of obesity in the adolescent population analyzed was observed. However, the 1858 CT genotype of the PTPN22 gene was associated with variations of certain biochemical parameters analyzed.

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