Target and resistance-related proteins of recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand on myeloma cell lines

  • Authors:
    • Yuan Jian
    • Yuling Chen
    • Chuanying Geng
    • Nian Liu
    • Guangzhong Yang
    • Jinwei Liu
    • Xin Li
    • Haiteng Deng
    • Wenming Chen
  • View Affiliations

  • Published online on: April 12, 2016     https://doi.org/10.3892/br.2016.650
  • Pages: 723-727
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Abstract

Recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand (rmhTRAIL) has become a potential therapeutic drug for multiple myeloma (MM). However, the exact targets and resistance mechanisms of rmhTRAIL on MM cells remain to be elucidated. The present study aimed to investigate the target and resistance‑related proteins of rmhTRAIL on myeloma cell lines. A TRAIL‑sensitive myeloma cell line, RPMI 8226, and a TRAIL‑resistance one, U266, were chosen and the differentially expressed proteins between the two cell lines were analyzed prior and subsequent to rmhTRAIL administration by a liquid chromatography‑tandem mass spectrometry method. The results showed that following TRAIL treatment, 6 apoptosis‑related proteins, calpain small subunit 1 (CPNS1), peflin (PEF1), B-cell receptor-associated protein 31 (BAP31), apoptosis‑associated speck‑like protein containing CARD (ASC), BAG family molecular chaperone regulator 2 (BAG2) and chromobox protein homolog 3 (CBX3), were upregulated in RPMI 8226 cells while no change was identified in the U266 cells. Furthermore, small ubiquitin‑related modifier 1 and several other ubiquitin proteasome pathway (UPP)‑related proteins expressed higher levels in TRAIL‑resistant cells U266 compared to the RPMI-8226 cells prior and subsequent to rmhTRAIL treatment. These results suggested that CPNS1, PEF1, BAP31, ASC, BAG2 and CBX3 were possibly target proteins of rmhTRAIL on RPMI 8226 cells, while UPP may have a vital role in mediating TRAIL‑resistance in U266 cells.
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June-2016
Volume 4 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Jian Y, Chen Y, Geng C, Liu N, Yang G, Liu J, Li X, Deng H and Chen W: Target and resistance-related proteins of recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand on myeloma cell lines. Biomed Rep 4: 723-727, 2016
APA
Jian, Y., Chen, Y., Geng, C., Liu, N., Yang, G., Liu, J. ... Chen, W. (2016). Target and resistance-related proteins of recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand on myeloma cell lines. Biomedical Reports, 4, 723-727. https://doi.org/10.3892/br.2016.650
MLA
Jian, Y., Chen, Y., Geng, C., Liu, N., Yang, G., Liu, J., Li, X., Deng, H., Chen, W."Target and resistance-related proteins of recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand on myeloma cell lines". Biomedical Reports 4.6 (2016): 723-727.
Chicago
Jian, Y., Chen, Y., Geng, C., Liu, N., Yang, G., Liu, J., Li, X., Deng, H., Chen, W."Target and resistance-related proteins of recombinant mutant human tumor necrosis factor-related apoptosis-inducing ligand on myeloma cell lines". Biomedical Reports 4, no. 6 (2016): 723-727. https://doi.org/10.3892/br.2016.650