Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines

Alizadeh‑Navaei,Reza; Rafiei,Alireza; Abedian‑Kenari,Saeid; Asgarian‑Omran,Hossein; Valadan,Reza; Hedayatizadeh‑Omran,Akbar

doi:10.3892/br.2016.684

Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines

Authors:
- Reza Alizadeh‑Navaei
- Alireza Rafiei
- Saeid Abedian‑Kenari
- Hossein Asgarian‑Omran
- Reza Valadan
- Akbar Hedayatizadeh‑Omran
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Affiliations: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari 48175‑861, Iran, Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari 48175‑861, Iran
Published online on: May 20, 2016 https://doi.org/10.3892/br.2016.684
Pages: 130-132

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Abstract

Evaluating the expression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) may be useful for predicting the best models and achieving more accurate results in cancer research. Therefore, the aim of the present study was to analyze the LGR5 expression levels in different cell lines. Eight commonly used cell lines were assessed (COS‑7, NIH3T3, HEK293, VERO, HeLa, BHK, HepG2 and AGS). All the cell lines were cultured in RPMI‑1640 medium contain 10% fetal calf serum at 37˚C in humidified conditions with 5% CO2. According to the western blotting results, LGR5 was expressed in all cell lines. Densitometry results of LGR5 expression in the different cell lines showed that high LGR5 expression levels were apparent in BHK, AGS, VERO and NIH3T3 cell lines compared with the other cell lines. The results indicate that for the normal and cancer cell lines, BNK and AGS may be a better choice, respectively, for in vitro cancer studies.

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