Glycolysis-associated enzymes existing in the follicular lumen of the thyroid may interfere with energy metabolism

Huang,Huibin; Shi,Yaxiong; Cai,Huiyao; Liang,Bo; Duan,Honghong; Cai,Qingyan

doi:10.3892/br.2016.721

Glycolysis-associated enzymes existing in the follicular lumen of the thyroid may interfere with energy metabolism

Authors:
- Huibin Huang
- Yaxiong Shi
- Huiyao Cai
- Bo Liang
- Honghong Duan
- Qingyan Cai
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Affiliations: Department of Endocrinology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, Department of Gynecology and Obstetrics. The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China
Published online on: July 18, 2016 https://doi.org/10.3892/br.2016.721
Pages: 317-320
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Synthesis and storage of the thyroid hormone precursor, thyroglobulin (TG), occurs within the follicular lumen of the thyroid and the TG is then absorbed into cells for further processing before release into the blood. However, the mechanism of energy metabolism in the follicular lumen of the thyroid remains unknown. In the present study, the three dimensional structure of thyroid follicles was constructed using a primary culture of swine cells and the follicular protein was identified via matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Three glycolysis-associated enzymes, enolase, pyruvate kinase and phosphoglyceraldehyde dehydrogenase were identified in addition to TG. These results support the hypothesis that anaerobic glycolysis of glucose exists in the follicle and supports energy consumption for hormone synthesis.

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