Association of DNA methyltransferase polymorphisms with susceptibility to primary gouty arthritis

Zhong,Xiaowu; Peng,Yuanhong; Yao,Chengjiao; Qing,Yufeng; Yang,Qibin; Guo,Xiaolan; Xie,Wenguang; Zhao,Mingcai; Cai,Xiaoming; Zhou,Jing-Guo

doi:10.3892/br.2016.746

Association of DNA methyltransferase polymorphisms with susceptibility to primary gouty arthritis

Authors:
- Xiaowu Zhong
- Yuanhong Peng
- Chengjiao Yao
- Yufeng Qing
- Qibin Yang
- Xiaolan Guo
- Wenguang Xie
- Mingcai Zhao
- Xiaoming Cai
- Jing-Guo Zhou
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Affiliations: Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Rheumatology and Immunology of the Affiliated Hospital, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Medicine Research Center, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Biology, North Sichuan Medical College, Nanchong, Sichuan 637007, P.R. China, Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan 637007, P.R. China
Published online on: August 26, 2016 https://doi.org/10.3892/br.2016.746
Pages: 467-472

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Abstract

Gouty arthritis is the most common type of inflammatory and immune disease, and the prevalence and incidence of gout increases annually. Genetic variations in the DNA methyltransferases (DNMTs) gene have not, to the best of our knowledge, been reported to influence gene expression and to participate in the pathogenesis of gout. The aim of the present study was to investigate whether the DNMT1, DNMT3A and DNMT3B polymorphisms contribute to gout susceptibility. These polymorphisms were screened for in 336 gout patients and 306 healthy control subjects (from a South China population) for association with gout. The distribution frequencies of DNMT1 rs2228611 AA genotype (P=0.007) and A allele (P=0.002; odds ratio=1.508, 95% confidence interval=1.158-1.964) were found to be significantly increased in the gout patients when compared with those in the healthy control subjects. The rs1550117 in DNMT3A and rs2424913 in DNMT3B exhibited no significant associations with gout susceptibility between the patients and control subjects. These results demonstrated that the DNMT1 rs2228611 polymorphism may be involved in the pathogenesis of gout, while DNMT3A rs1550117 and DNMT3B rs2424913 did not show any obvious significance in the current study; thus, may not be used as risk factors to predict the susceptibility to gout. However, further studies are required to investigate the functions and regulatory mechanism of the polymorphisms of DNMTs in gout.

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