Resistin-induced cardiomyocyte hypertrophy is inhibited by apelin through the inactivation of extracellular signal-regulated kinase signaling pathway in H9c2 embryonic rat cardiomyocytes

  • Authors:
    • Jian‑Wei Luo
    • Xian Zheng
    • Guan‑Chang Cheng
    • Qun‑Hui Ye
    • Yong‑Zhi Deng
    • Lin Wu
  • View Affiliations

  • Published online on: September 2, 2016     https://doi.org/10.3892/br.2016.749
  • Pages: 473-478
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Abstract

It has been reported that resistin induces, whereas apelin inhibits cardiac hypertrophy. However, the underlying molecular mechanisms of apelin inhibiting resistin-induced cardiac hypertrophy remain unclear. The aim of the current study is to investigate the effects of apelin on resistin‑induced cardiomyocyte hypertrophy and elucidate the underlying molecular mechanism. H9c2 cells were used in the present study, and cell surface area and protein synthesis were evaluated. Reverse transcription‑quantitative polymerase chain reaction was performed to analyze the expression levels of hypertrophic markers, brain natriuretic peptide (BNP) and β‑myosin heavy chain (β‑MHC). In addition, western blotting was conducted to examine phosphorylation of extracellular signal‑regulated kinase (ERK)1/2. Following treatment of H9c2 cells with resistin, cell surface area, protein synthesis, and BNP and β‑MHC mRNA expression levels were increased. Subsequent to co‑treatment of H9c2 cells with apelin and resistin, lead to the inhibition of resistin‑induced hypertrophic effects by apelin. In addition, treatment with resistin increased phosphorylation of ERK1/2, whereas pretreatment with apelin decreased phosphorylation of ERK1/2, which was increased by resistin. These results indicate that resistin-induced cardiac hypertrophy is inhibited by apelin via inactivation of ERK1/2 cell signaling.
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October-2016
Volume 5 Issue 4

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Luo JW, Zheng X, Cheng GC, Ye QH, Deng YZ and Wu L: Resistin-induced cardiomyocyte hypertrophy is inhibited by apelin through the inactivation of extracellular signal-regulated kinase signaling pathway in H9c2 embryonic rat cardiomyocytes. Biomed Rep 5: 473-478, 2016.
APA
Luo, J., Zheng, X., Cheng, G., Ye, Q., Deng, Y., & Wu, L. (2016). Resistin-induced cardiomyocyte hypertrophy is inhibited by apelin through the inactivation of extracellular signal-regulated kinase signaling pathway in H9c2 embryonic rat cardiomyocytes. Biomedical Reports, 5, 473-478. https://doi.org/10.3892/br.2016.749
MLA
Luo, J., Zheng, X., Cheng, G., Ye, Q., Deng, Y., Wu, L."Resistin-induced cardiomyocyte hypertrophy is inhibited by apelin through the inactivation of extracellular signal-regulated kinase signaling pathway in H9c2 embryonic rat cardiomyocytes". Biomedical Reports 5.4 (2016): 473-478.
Chicago
Luo, J., Zheng, X., Cheng, G., Ye, Q., Deng, Y., Wu, L."Resistin-induced cardiomyocyte hypertrophy is inhibited by apelin through the inactivation of extracellular signal-regulated kinase signaling pathway in H9c2 embryonic rat cardiomyocytes". Biomedical Reports 5, no. 4 (2016): 473-478. https://doi.org/10.3892/br.2016.749