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Article

Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells

  • Authors:
    • Chun‑Peng Zhao
    • Zhong‑Jie Xu
    • Qing Guo
    • Yun‑Xiao Li
    • Xiang‑Zheng Gao
    • Yi‑You Peng
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of College of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
  • Pages: 585-588
    |
    Published online on: September 21, 2016
       https://doi.org/10.3892/br.2016.759
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Abstract

In a previous study, the suppressor of IKBKE 1 expression level was confirmed to be higher in vincristine (VCR)‑resistant HCT‑8 (HCT‑8/V) colon cancer cells than in non‑VCR‑resistant HCT‑8 cells. In the current study, IKBKE 1 expression in VCR‑resistant colon cancer cells was investigated further. HCT‑8 and HCT‑8/V human colon cancer cells were used, and polymerase chain reaction (PCR) primers were designed to amplify the IKBKE 1 gene. Fluorescence reverse transcription‑quantitative PCR (RT‑qPCR) was performed to detect differences in IKBKE 1 expression between sensitive and drug‑resistant colon cancer cell lines. Western blotting was performed to further observe IKBKE 1 expression. Based on the RT‑qPCR and western blot results, IKBKE 1 expression was observed to be markedly higher in the HCT‑8/V cells, and this difference was significant (P<0.05). Thus, IKBKE 1 expression was identified to be associated with the resistance of colon cancer cells to VCR.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao CP, Xu ZJ, Guo Q, Li YX, Gao XZ and Peng YY: Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells. Biomed Rep 5: 585-588, 2016.
APA
Zhao, C., Xu, Z., Guo, Q., Li, Y., Gao, X., & Peng, Y. (2016). Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells. Biomedical Reports, 5, 585-588. https://doi.org/10.3892/br.2016.759
MLA
Zhao, C., Xu, Z., Guo, Q., Li, Y., Gao, X., Peng, Y."Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells". Biomedical Reports 5.5 (2016): 585-588.
Chicago
Zhao, C., Xu, Z., Guo, Q., Li, Y., Gao, X., Peng, Y."Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells". Biomedical Reports 5, no. 5 (2016): 585-588. https://doi.org/10.3892/br.2016.759
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao CP, Xu ZJ, Guo Q, Li YX, Gao XZ and Peng YY: Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells. Biomed Rep 5: 585-588, 2016.
APA
Zhao, C., Xu, Z., Guo, Q., Li, Y., Gao, X., & Peng, Y. (2016). Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells. Biomedical Reports, 5, 585-588. https://doi.org/10.3892/br.2016.759
MLA
Zhao, C., Xu, Z., Guo, Q., Li, Y., Gao, X., Peng, Y."Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells". Biomedical Reports 5.5 (2016): 585-588.
Chicago
Zhao, C., Xu, Z., Guo, Q., Li, Y., Gao, X., Peng, Y."Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells". Biomedical Reports 5, no. 5 (2016): 585-588. https://doi.org/10.3892/br.2016.759
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