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Article

Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12

  • Authors:
    • Zhi Guo
    • Hong‑Yan Gao
    • Tian‑Yang Zhang
    • Jin‑Xing Lou
    • Kai Yang
    • Xiao‑Dong Liu
    • Xue‑Peng He
    • Hui‑Ren Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Beijing Military General Hospital, Beijing 100700, P.R. China
  • Pages: 567-573
    |
    Published online on: October 6, 2016
       https://doi.org/10.3892/br.2016.773
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Abstract

The aim of the present study was to construct a chimeric adenovirus (Ad)5/F35 co-expressing human CD4O ligand (CD4OL) and interleukin (IL)‑2 (Ad5/F35 CD40L‑IL‑2). The infection efficiency to human monocyte‑derived dendritic cells (Mo‑DCs), expression of genes, phenotype changes and IL‑12 production of Mo‑DC by Ad5/F35 CD40L‑IL‑2 were investigated. CD40L and IL‑2 from total RNA extracted from human peripheral blood mononuclear cells (PBMCs) were cloned by reverse transcription‑polymerase chain reaction and used to construct Ad5/F35 CD40L‑IL‑2. The infection efficiency, expression of CD40L, and phenotype changes of Mo‑DC infected with Ad5/F35 CD40L‑IL‑2 were analyzed using flow cytometry. The quantities of IL‑2 and IL‑12 in the supernatants of Mo‑DC following infection of Ad5/F35 CD40L‑IL‑2 were measured by enzyme‑linked immunosorbent assay. The CD40L and IL‑2 genes were successfully cloned and the Ad5/F35 CD40L‑IL‑2 was constructed. Ad5/F35 CD40L‑IL‑2 efficiently infected Mo‑DCs with an infection efficiency of >75%, and the infected Mo‑DCs expressed CD40L and secreted IL‑2. The expression levels of cluster of differentiation (CD)80, CD86, CD40, and human leukocyte antigen‑antigen D related on Mo‑DC were moderate; however, CD83 was low prior to infection of Ad5/F35 CD40L‑IL‑2. Those molecules, particularly CD83, were markedly upregulated 24 h after the infection. Increasing quantities of IL‑12 in the supernatants were detected subsequent to infection at different time points in a time‑dependent manner. Thus, Ad5/F35 CD40L‑IL‑2 efficiently infected human Mo‑DCs and its products, CD40L and IL‑2, were subsequently expressed. In addition, infection with Ad5/F35 CD40L‑IL‑2 stimulated the maturation of Mo‑DC and high levels of IL‑12 production.
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Copy and paste a formatted citation
Spandidos Publications style
Guo Z, Gao HY, Zhang TY, Lou JX, Yang K, Liu XD, He XP and Chen HR: Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12. Biomed Rep 5: 567-573, 2016.
APA
Guo, Z., Gao, H., Zhang, T., Lou, J., Yang, K., Liu, X. ... Chen, H. (2016). Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12. Biomedical Reports, 5, 567-573. https://doi.org/10.3892/br.2016.773
MLA
Guo, Z., Gao, H., Zhang, T., Lou, J., Yang, K., Liu, X., He, X., Chen, H."Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12". Biomedical Reports 5.5 (2016): 567-573.
Chicago
Guo, Z., Gao, H., Zhang, T., Lou, J., Yang, K., Liu, X., He, X., Chen, H."Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12". Biomedical Reports 5, no. 5 (2016): 567-573. https://doi.org/10.3892/br.2016.773
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Z, Gao HY, Zhang TY, Lou JX, Yang K, Liu XD, He XP and Chen HR: Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12. Biomed Rep 5: 567-573, 2016.
APA
Guo, Z., Gao, H., Zhang, T., Lou, J., Yang, K., Liu, X. ... Chen, H. (2016). Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12. Biomedical Reports, 5, 567-573. https://doi.org/10.3892/br.2016.773
MLA
Guo, Z., Gao, H., Zhang, T., Lou, J., Yang, K., Liu, X., He, X., Chen, H."Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12". Biomedical Reports 5.5 (2016): 567-573.
Chicago
Guo, Z., Gao, H., Zhang, T., Lou, J., Yang, K., Liu, X., He, X., Chen, H."Adenovirus co‑expressing CD40 ligand and interleukin (IL)‑2 contributes to maturation of dendritic cells and production of IL‑12". Biomedical Reports 5, no. 5 (2016): 567-573. https://doi.org/10.3892/br.2016.773
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