Ex vivo regenerative effects of a spring water

Nicoletti,Giovanni; Saler,Marco; Pellegatta,Tommaso; Tresoldi,Marco  Mario; Bonfanti,Viola; Malovini,Alberto; Faga,Angela; Riva,Federica

doi:10.3892/br.2017.1002

Ex vivo regenerative effects of a spring water

Authors:
- Giovanni Nicoletti
- Marco Saler
- Tommaso Pellegatta
- Marco Mario Tresoldi
- Viola Bonfanti
- Alberto Malovini
- Angela Faga
- Federica Riva
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Affiliations: Plastic and Reconstructive Surgery, Department of Clinical‑Surgical Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, Italy, Laboratory of Informatics and Systems Engineering for Clinical Research, Istituti Clinici Scientifici Maugeri, 27100 Pavia, Italy, Advanced Technologies for Regenerative Medicine and Inductive Surgery Research Center, University of Pavia, 27100 Pavia, Italy
Published online on: October 18, 2017 https://doi.org/10.3892/br.2017.1002
Pages: 508-514
Copyright: © Nicoletti et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous experiments by our group have indicated the regenerative effects of a spring water (Comano), which was possibly associated with the native non‑pathogenic bacterial flora. The present study aimed to confirm these regenerative properties in a human ex vivo experimental model in the context of physiological wound healing. Human 6‑mm punch skin biopsies harvested during plastic surgery sessions were injured in their central portion to induce skin loss and were cultured in either conventional medium (controls) or medium powder reconstituted with filtered Comano spring water (treated samples). At 24, 48 and 72 h the specimens were observed following staining with hematoxylin and eosin, Picrosirius Red, orcein and anti‑proliferating cell nuclear antigen. Compared with the controls, the treated samples exhibited reduced overall cell infiltration, evidence of fibroblasts, stimulation of cell proliferation and collagen and elastic fiber regeneration. In the spring water, in addition to 12 resident non‑pathogenic bacterial strains exhibiting favorable metabolic activities, more unknown non‑pathogenic species are being identified by genomic analysis. In the present study, the efficacy of this ‘germ‑free’, filtered spring water in wound regeneration was indicated. Thus, the Comano spring water microbiota should be acknowledged for its regenerative properties.

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