Potential of bone marrow mesenchymal stem cells in rejuvenation of the aged skin of rats

Liu,Zhen; Hu,Guo‑Dong; Luo,Xiao‑Bo; Yin,Bin; Shu,Bin; Guan,Jing‑Zhi; Jia,Chi‑Yu

doi:10.3892/br.2017.842

Potential of bone marrow mesenchymal stem cells in rejuvenation of the aged skin of rats

Authors:
- Zhen Liu
- Guo‑Dong Hu
- Xiao‑Bo Luo
- Bin Yin
- Bin Shu
- Jing‑Zhi Guan
- Chi‑Yu Jia
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Affiliations: Department of Burns and Plastic Surgery, The 309th Hospital of PLA, Beijing 100091, P.R. China, Department of Orthopedics, The 309th Hospital of PLA, Beijing 100091, P.R. China, Department of Oncology, The 309th Hospital of PLA, Beijing 100091, P.R. China
Published online on: January 13, 2017 https://doi.org/10.3892/br.2017.842
Pages: 279-284
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to evaluate the anti‑aging effects of bone marrow‑mesenchymal stem cells (BM‑MSCs) in a D‑galactose-induced skin aging rat model. Male Sprague Dawley rats were randomly divided into four groups (n=10/group) as follows: Normal control group; skin aging model group; MSC‑treated group by subcutaneous multi‑point injection. The skin aging model was established by a daily subcutaneous injection of 15% D‑galactose (1,000 mg/kg) for 8 weeks. Rats in the MSC‑treated groups were administered 3x106/ml BM‑MSCs/green fluorescent protein (GFP) for 4 weeks, administered once per week. Oxidative/antioxidative parameters were evaluated, and morphological and ultrastructure analyses were performed. Rats in the model group exhibited the typical changes of aging skin. Compared with the control group, rats in the model group had significantly increased malondialdehyde (MDA) content (P<0.01), and decreased serum superoxide dismutase (SOD) and glutathione peroxidase (GSH‑Px) activities (P<0.05). MSC treatment markedly ameliorated aging-induced oxidative stress in the skin. Histologically, rats in the model group exhibited loosely arranged epidermal cell layers and disorganized collagen fibers. BM‑MSC treatment significantly improved the histological abnormalities, which was similar to those in the control group. In addition, 7 days after the final cell transplantation, GFP‑positive cells were observed by fluorescence microscopy to be distributed in the dermis. Injection of BM‑MSCs significantly improved the D‑galactose‑induced histological abnormalities of the skin, by promoting an antioxidant response and ameliorating oxidative stress in aged skin. Thus, BM‑MSCs may be beneficial in the rejuvenation of aged skin.

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