Biocompatibility of biological material polylactic acid with stem cells from human exfoliated deciduous teeth

Wang,Xi; Li,Guanghui; Liu,Yiming; Yu,Weiwei; Sun,Qiang

doi:10.3892/br.2017.881

Biocompatibility of biological material polylactic acid with stem cells from human exfoliated deciduous teeth

Authors:
- Xi Wang
- Guanghui Li
- Yiming Liu
- Weiwei Yu
- Qiang Sun
View Affiliations

Affiliations: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
Published online on: March 28, 2017 https://doi.org/10.3892/br.2017.881
Pages: 519-524
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

To investigate the biocompatibility of the biomaterial, polylactic acid (PLA) with stem cells from human exfoliated deciduous teeth (SHED) and its induction of mineralization as a type of scaffold material. To determine the impacts of biomaterial PLA on proliferation and mineralization of SHED, the expression of surface molecules of SHED isolated and cultured in vitro was detected by flow cytometry. In addition, cell proliferation was measured using MTT and Edu assays, and the evaluation of mineralized differentiation was performed using Alizarin Red S staining. In addition, the expression levels of osteogenic marker genes were measured by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis. SHED were successfully isolated and identified. The MTT and Edu results indicated that the proliferation of SHED cultured in PLA and normal medium was not significantly different. The Alizarin Red S staining demonstrated that the mineralization capability was significantly higher in the SHED that were cultured in PLA medium. Furthermore, RT‑qPCR and western blot analyses indicated that the expression levels of osteogenic marker genes were higher in the SHED cultured in PLA medium. These results suggested that PLA possesses good biocompatibility with SHED and may effectively induce the mineralization of SHED and serve as a scaffold material.

View Figures

View References

1	Duailibi MT, Duailibi SE, Young CS, Bartlett JD, Vacanti JP and Yelick PC: Bioengineered teeth from cultured rat tooth bud cells. J Dent Res. 83:523–528. 2004. View Article : Google Scholar : PubMed/NCBI
2	Young CS, Terada S, Vacanti JP, Honda M, Bartlett JD and Yelick PC: Tissue engineering of complex tooth structures on biodegradable polymer scaffolds. J Dent Res. 81:695–700. 2002. View Article : Google Scholar : PubMed/NCBI
3	Liu X, Holzwarth JM and Ma PX: Functionalized synthetic biodegradable polymer scaffolds for tissue engineering. Macromol Biosci. 12:911–919. 2012. View Article : Google Scholar : PubMed/NCBI
4	Gao W and Wang J: Synthetic micro/nanomotors in drug delivery. Nanoscale. 6:10486–10494. 2014. View Article : Google Scholar : PubMed/NCBI
5	Wiebe J, Nef HM and Hamm CW: Current status of bioresorbable scaffolds in the treatment of coronary artery disease. J Am Coll Cardiol. 64:2541–2551. 2014. View Article : Google Scholar : PubMed/NCBI
6	Vanaman M and Fabi SG: Décolletage: Regional Approaches with Injectable Fillers. Plast Reconstr Surg. 136:276S–281S. 2015. View Article : Google Scholar : PubMed/NCBI
7	Gentile P, Chiono V, Carmagnola I and Hatton PV: An overview of poly(lactic-co-glycolic) acid (PLGA)-based biomaterials for bone tissue engineering. Int J Mol Sci. 15:3640–3659. 2014. View Article : Google Scholar : PubMed/NCBI
8	Venkatesan J and Kim SK: Nano-hydroxyapatite composite biomaterials for bone tissue engineering--a review. J Biomed Nanotechnol. 10:3124–3140. 2014. View Article : Google Scholar : PubMed/NCBI
9	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
10	Langer R and Vacanti JP: Tissue engineering. Science. 260:920–926. 1993. View Article : Google Scholar : PubMed/NCBI
11	Jeong W, Shin EJ, Culkin DA, Hedrick JL and Waymouth RM: Zwitterionic polymerization: A kinetic strategy for the controlled synthesis of cyclic polylactide. J Am Chem Soc. 131:4884–4891. 2009. View Article : Google Scholar : PubMed/NCBI
12	Castro-Aguirre E, Iñiguez-Franco F, Samsudin H, Fang X and Auras R: Poly(lactic acid)-Mass production, processing, industrial applications, and end of life. Adv Drug Deliv Rev. 107:333–366. 2016. View Article : Google Scholar : PubMed/NCBI
13	Sudwilai T, Ng JJ, Boonkrai C, Israsena N, Chuangchote S and Supaphol P: Polypyrrole-coated electrospun poly(lactic acid) fibrous scaffold: Effects of coating on electrical conductivity and neural cell growth. J Biomater Sci Polym Ed. 25:1240–1252. 2014. View Article : Google Scholar : PubMed/NCBI
14	Vuornos K, Björninen M, Talvitie E, Paakinaho K, Kellomäki M, Huhtala H, Miettinen S, Seppänen-Kaijansinkko R and Haimi S: Human Adipose Stem Cells Differentiated on Braided Polylactide Scaffolds Is a Potential Approach for Tendon Tissue Engineering. Tissue Eng Part A. 22:513–523. 2016. View Article : Google Scholar : PubMed/NCBI
15	Kim IG, Hwang MP, Du P, Ko J, Ha CW, Do SH and Park K: Bioactive cell-derived matrices combined with polymer mesh scaffold for osteogenesis and bone healing. Biomaterials. 50:75–86. 2015. View Article : Google Scholar : PubMed/NCBI
16	Salerno A, Guarino V, Oliviero O, Ambrosio L and Domingo C: Bio-safe processing of polylactic-co-caprolactone and polylactic acid blends to fabricate fibrous porous scaffolds for in vitro mesenchymal stem cells adhesion and proliferation. Mater Sci Eng C. 63:512–521. 2016. View Article : Google Scholar
17	Gronthos S, Mankani M, Brahim J, Robey PG and Shi S: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 97:13625–13630. 2000. View Article : Google Scholar : PubMed/NCBI
18	Shi S and Gronthos S: Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res. 18:696–704. 2003. View Article : Google Scholar : PubMed/NCBI
19	Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG and Shi S: SHED: Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA. 100:5807–5812. 2003. View Article : Google Scholar : PubMed/NCBI
20	Galler KM, Cavender A, Yuwono V, Dong H, Shi S, Schmalz G, Hartgerink JD and D'Souza RN: Self-assembling peptide amphiphile nanofibers as a scaffold for dental stem cells. Tissue Eng Part A. 14:2051–2058. 2008. View Article : Google Scholar : PubMed/NCBI
21	Cordeiro MM, Dong Z, Kaneko T, Zhang Z, Miyazawa M, Shi S, Smith AJ and Nör JE: Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. J Endod. 34:962–969. 2008. View Article : Google Scholar : PubMed/NCBI
22	Yamaza T, Miura Y, Bi Y, Liu Y, Akiyama K, Sonoyama W, Patel V, Gutkind S, Young M, Gronthos S, et al: Pharmacologic stem cell based intervention as a new approach to osteoporosis treatment in rodents. PLoS One. 3:e26152008. View Article : Google Scholar : PubMed/NCBI