Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury

Gong,Jianhua; Wang,Junyi; Tian,Yu; Zhang,Jing; Liang,Wenjin; Li,Zeming; Yu,Jidong; Tang,Bo; He,Songqing

doi:10.3892/br.2017.891

Expression of tubulin folding cofactor B in mouse hepatic ischemia‑reperfusion injury

Authors:
- Jianhua Gong
- Junyi Wang
- Yu Tian
- Jing Zhang
- Wenjin Liang
- Zeming Li
- Jidong Yu
- Bo Tang
- Songqing He
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Affiliations: Department of Hepatobiliary Surgery and Laboratory, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China, Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The Second Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. China
Published online on: April 11, 2017 https://doi.org/10.3892/br.2017.891
Pages: 525-531
Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the association between tubulin folding cofactor B (TBCB) expression and ischemia‑reperfusion injury (IRI) in mice. A total of 48 C57BL/6 mice were randomly divided into a control group (Sham, n=6) and an ischemia‑reperfusion group (n=42). The ischemia‑reperfusion group was further divided into 6 subgroups as per different times after reperfusion (2, 4, 6, 8, 12 and 24 h), with 7 mice per subgroup. A hepatic IRI model was established in mice by clamping the hepatic hilum. Morphology, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin 6 (IL‑6) and tumor necrosis factor‑α (TNF‑α), and the expression level of TBCB were detected. Compared with the control group, the livers from the ischemia‑reperfusion group were significantly changed, particularly at 12 h following ischemia‑reperfusion, with obvious hepatic cell degeneration and necrosis. The ALT, AST, IL‑6 and TNF‑α levels in the sera of the mice in the hepatic ischemia‑reperfusion group were increased at all time points following ischemia‑reperfusion, and were the highest at 12 h, demonstrating statistically significant differences when compared with the control group (P<0.05). Furthermore, the expression levels of TBCB, TNF‑α and IL‑6 were significantly increased at all time-points following ischemia‑reperfusion, and were the most significant at 12 h. At 24 h following ischemia‑reperfusion, the expression levels had decreased. The present study indicated that TBCB expression is associated with TNF‑α and IL‑6 expression levels in mice with hepatic ischemia‑reperfusion, and may be key in the development of liver injury during ischemia‑reperfusion in mice.

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