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Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study

  • Authors:
    • Luyang Zhao
    • Chenglei Gu
    • Mingxia Ye
    • Zhe Zhang
    • Weidong Han
    • Wensheng Fan
    • Yuanguang Meng
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, People's Liberation Army Medical School, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China, Department of Molecular Biology, Institute of Basic Medicine, People's Liberation Army Medical School, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 654-662
    |
    Published online on: May 3, 2017
       https://doi.org/10.3892/br.2017.902
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Abstract

The etiology and pathophysiology of endometriosis remain unclear. The aim of the current study was to identify a candidate pathogenic gene, as well as potential biomarkers of endometriosis using messenger RNA (mRNA) sequencing (mRNA-seq). Twenty-three eutopic endometria from women with endometriosis and 20 endometria from control subjects were investigated. Eight eutopic endometria and five normal endometria were selected for mRNA‑seq. Differentially expressed genes (DEGs) were identified and functional analysis was conducted. Validation of certain DEGs was performed in the remaining cases and control subjects by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). A total of 72 DEGs (66 upregulated and 6 downregulated) were identified in samples from women with endometriosis and compared with the control subjects. High DEGs included those involved in various functions, such as extracellular matrix (ECM) remodeling, angiogenesis, cell proliferation and differentiation. Enriched by these DEGs, 100 Gene Ontology terms were identified as significantly important, particularly ‘ECM’ and ‘endogenous stimulus’. Validation using RT‑qPCR indicated that matrix metallopeptidase 11, dual specificity phosphatase 1, Fos proto‑oncogeneand serpin family E member 1 were significantly upregulated and adenosine deaminase 2 was significantly downregulated in the eutopic endometrium of patients with endometriosis. The identified DEGs may be involved in the pathogenesis of endometriosis and may be potential biomarkers in the eutopic endometrium. The current study provides a comprehensive, but preliminary insight for elucidating the mechanisms of endometriosis, which require further in‑depth studies for confirmation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao L, Gu C, Ye M, Zhang Z, Han W, Fan W and Meng Y: Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study. Biomed Rep 6: 654-662, 2017.
APA
Zhao, L., Gu, C., Ye, M., Zhang, Z., Han, W., Fan, W., & Meng, Y. (2017). Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study. Biomedical Reports, 6, 654-662. https://doi.org/10.3892/br.2017.902
MLA
Zhao, L., Gu, C., Ye, M., Zhang, Z., Han, W., Fan, W., Meng, Y."Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study". Biomedical Reports 6.6 (2017): 654-662.
Chicago
Zhao, L., Gu, C., Ye, M., Zhang, Z., Han, W., Fan, W., Meng, Y."Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study". Biomedical Reports 6, no. 6 (2017): 654-662. https://doi.org/10.3892/br.2017.902
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao L, Gu C, Ye M, Zhang Z, Han W, Fan W and Meng Y: Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study. Biomed Rep 6: 654-662, 2017.
APA
Zhao, L., Gu, C., Ye, M., Zhang, Z., Han, W., Fan, W., & Meng, Y. (2017). Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study. Biomedical Reports, 6, 654-662. https://doi.org/10.3892/br.2017.902
MLA
Zhao, L., Gu, C., Ye, M., Zhang, Z., Han, W., Fan, W., Meng, Y."Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study". Biomedical Reports 6.6 (2017): 654-662.
Chicago
Zhao, L., Gu, C., Ye, M., Zhang, Z., Han, W., Fan, W., Meng, Y."Identification of global transcriptome abnormalities and potential biomarkers in eutopic endometria of women with endometriosis: A preliminary study". Biomedical Reports 6, no. 6 (2017): 654-662. https://doi.org/10.3892/br.2017.902
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