Downregulating forkhead box M1 inhibits proliferation by inhibiting autophagy in the sw480 cell line

Zhong,Shibiao; Zhou,Aiyan; Qi,Fanghua; Li,Zhen; Yu,Zeyan; Lu,Yongchao; Liu,Xudong

doi:10.3892/br.2017.915

Downregulating forkhead box M1 inhibits proliferation by inhibiting autophagy in the sw480 cell line

Authors:
- Shibiao Zhong
- Aiyan Zhou
- Fanghua Qi
- Zhen Li
- Zeyan Yu
- Yongchao Lu
- Xudong Liu
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Affiliations: Department of Anorectal Surgery, Minzu Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi Zhuang Autonomous Region 530001, P.R. China, Department of Traditional Chinese Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 510080, P.R. China, Department of Pain, Qilu Hospital of Shandong University, Jinan, Shandong 250000, P.R. China
Published online on: May 19, 2017 https://doi.org/10.3892/br.2017.915
Pages: 47-50

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Abstract

Forkhead Box M1 (FoxM1) is one of the most important oncogenes, and overexpression of FoxM1 has been reported in many cancers, including colon cancer. In the present study, the authors attempted to reveal the mechanism underlying its effects on proliferation through autophagy in the sw480 cell line. FoxM1 is knocked down through short hairpin (sh)RNA in the sw480 cell line. A series of experiments were conducted to examine it function on proliferation and LC3 and P62 were used to measure level of autophagy. Autophagy in the shFoxM1 cell was demonstrated as significantly inhibited compared with the negative control. Additional auto‑fluex was also tested, downregulation of FoxM1 served the same role as BA1 in autophagy. Furthermore, downregulating FoxM1 inhibited cell proliferation in the sw480 cell line.

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