The pattern of IL‑24/mda‑7 and its cognate receptors expression following activation of human hepatic stellate cells

Jamhiri,Iman; Hosseini,Seyed Younes; Mehrabani,Davood; Khodabandeh,Zahra; Yaghobi,Ramin; Dowran,Razieh; Zahri,Saber

doi:10.3892/br.2017.931

The pattern of IL‑24/mda‑7 and its cognate receptors expression following activation of human hepatic stellate cells

Authors:
- Iman Jamhiri
- Seyed Younes Hosseini
- Davood Mehrabani
- Zahra Khodabandeh
- Ramin Yaghobi
- Razieh Dowran
- Saber Zahri
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Affiliations: Department of Biology, Cell and Molecular Laboratory, Faculty of Science, University of Mohaghegh Ardabili, Ardabil 56199‑11367, Iran, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz 71348‑14336, Iran, Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz 71348‑14336, Iran, Transplant Research Center, Shiraz University of Medical Sciences, Shiraz 71348‑14336, Iran
Published online on: June 21, 2017 https://doi.org/10.3892/br.2017.931
Pages: 173-178

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Abstract

Activation of hepatic stellate cells (HSCs) is the pivotal event during liver fibrosis. Interleukin (IL)‑24/melanoma differentiation‑associated gene‑7 (mda‑7) has attracted attention in the pathophysiology of some diseases, while its role in activation/suppression of human HSCs is still unclear. It is important to elucidate whether the expression levels of the IL‑24/mda‑7 protein and its receptors in HSC cells are changed following activation. LX‑2 cells, a human hepatic stellate cell line were activated by a combination of leptin and serum starvation. The activation state was evaluated through measuring the mRNA expression of profibrotic molecules, collagen‑I, TIMP metalloproteinase inhibitor‑1 and transforming growth factor‑β. The expression of IL‑24/mda‑7 was assessed in mRNA and protein levels by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and ELISA methods, respectively. Hence, the amount of IL‑22R1 and IL‑20R2 subunit expression was also compared in activated and normal LX‑2 cells by RT‑qPCR. The expression level of IL‑24/mda‑7 and its cognate receptors was detectable both in the normal and activated LX‑2 cell line. Furthermore, in activated LX‑2, a significant increase of IL24 expression either on IL‑22R1 and IL‑20R2 subunits was also noticeable in comparison to normal cells. The activation state of LX‑2 cells caused significant changes of IL‑24/mda‑7 and its receptors expression. In addition, the elevation in IL‑24/mda‑7 during LX‑2 cell activation, suggested that IL‑24/mda‑7 and its cognate receptors serve a possible role in the development of the fibrosis process. Therefore, IL‑24/mda‑7 and relevant signaling pathways may be employed as a target for fibrosis treatment.

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