Long-term rearrangement of retinal structures in a novel mutation of X-linked retinoschisis

  • Authors:
    • Stefano Piermarocchi
    • Stefania Miotto
    • Davide Colavito
    • Elda Del Giudice
    • Alberta Leon
    • Veronica Maritan
    • Rita Piermarocchi
    • Alma Patrizia Tormene
  • View Affiliations

  • Published online on: July 27, 2017     https://doi.org/10.3892/br.2017.954
  • Pages: 241-246
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to report a novel mutation in the retinoschisin 1 (RS1) gene in a Caucasian family affected by X-linked juvenile retinoschisis (XLRS) and to describe the long-term modification of retinal structure. Two brothers with an early onset maculopathy were diagnosed with XLRS. Fundus photography, fluorescein angiography, spectral domain optical coherence tomography and electroretinogram analyses were performed. Their sister was also examined. All subjects were screened for mutations in the RS1 gene. XLRS patients demonstrated a marked reduction of best-corrected visual acuity. SD-OCT scans reported a cystic degeneration primarily involving the inner nuclear layer, though some cysts were detected in the outer plexiform layer and in the ganglion cell layer. During the ten-year follow-up, a progressive retinal thickening and coalescence of the cysts was observed. Genetic testing revealed a novel mutation (p.Ile212Asn) in the RS1 gene in both XLRS patients, whereas their sister was not a genetic carrier. Several mutations of the RS1 gene were recognized to be responsible for XLRS. Although the correspondence between genotype and phenotype is still under debate, is reasonable that siblings affected by XLRS could share other genetic and/or epigenetic factors capable to influence clinical course of the disease.

Introduction

X-linked juvenile retinoschisis (XLRS, phenotype MIM 312700), first described in 1898 by Joseph Haas, represents the leading cause of early macular degeneration in males (1,2). Its prevalence ranges from 1:5,000 to 1:25,000 (3). Bilateral splitting of the inner retina is the most common clinical finding and has been reported in 68–100% of affected males. In ophthalmoscopy, the macula presents stellate spoke-wheel pattern. Female carriers rarely present with retinal abnormalities (4). As the disease progresses, retinal cysts often coalesce with subsequent retinal flattening and macular atrophy in older patients. Peripheral schisis, usually located in the inferotemporal retina, is evident in ~50% of the affected individuals (5). Although this condition is considered to be congenital, symptoms generally present in the first decade of life with visual failure, squint or nystagmus. Visual damage usually progresses in the first two decades of life and remains approximately stationary until the fifth or sixth decade, when the development of macular atrophy induces additional visual loss (3).

Patients with XLRS present a characteristic pattern on the electroretinogram (ERG). A reduction in the amplitude of the b-wave (generated by the activity of depolarising bipolar cells) and a relative preservation of the negative a-wave (generated by photoreceptors) give rise to, the so-called electronegative ERG (6). The absence of negative ERG does not necessarily exclude the presence of the pathology: in a series of 24 XLRS patients, only 56.5% presented with typical negative ERG (7).

Optical coherence tomography (OCT) is a key diagnostic test for XLRS. Schisis seems to occur predominantly at the inner nuclear layer (INL), occasionally at the outer nuclear layer/outer plexiform layer (ONL/OPL), and only rarely at the retinal nerve fiber layer (RNFL). The schisis cavity may extend beyond the retinal vascular arcades (8).

The gene responsible for XLRS, retinoschisin 1 (RS1), was identified by Sauer et al (2) in 1997. The RS1 gene, on chromosome Xp22, encodes for retinoschisin, a discoidin-domain containing protein, which is secreted by photoreceptors and bipolar cells as a homo-oligomeric complex (2,9,10). Retinoschisin complex binds tightly to the surface of photoreceptors and bipolar cells, contributing to maintain the structural organization of the retina and of the photoreceptor-bipolar synapse (4). In people with XLRS, several missense and nonsense mutations, insertion and deletion mutations, intragenic deletions and splice site mutations have been identified (11).

The aim of the present study is to describe clinical features of a family affected by the novel missense mutation I212N of the RS gene and to provide a detailed follow-up based on spectral domain optical coherence tomography (SD-OCT).

Materials and methods

Patients

The present study included a Caucasian Italian family with two brothers clinically affected by XLRS. Both patients underwent a complete ophthalmological assessment and were followed up for ten years, including best-corrected visual acuity (BCVA) and fundus examination. Detailed multimodal retinal imaging (fundus photography, OCT, fundus autofluorescence (FAF) and retinal angiography) was collected. Moreover, retinal function was evaluated with ERG and Goldmann perimetry. Clinical and genetic testing was extended to other family members. The current study was conducted in accordance with The Declaration of Helsinki.

XLRS1 gene analysis

Written informed consent for genetic analysis was obtained from the all subjects. Whole blood samples were then collected. DNA was extracted using the Qiagen Biorobot DNA extraction kit (Qiagen, Inc., Valencia, CA, USA) according to manufacturer's instructions and quantified by Nanodrop spectral analysis (Thermo Fisher Scientific, Inc., Waltham, MA, USA). DNA fragmentation and degradation were evaluated by standard agarose gel electrophoresis. A total of 100 ng DNA were amplified by standard polymerase chain reaction (PCR) procedures with a PCR mixture containing 2.5 µl 10X concentrated PCR buffer (Solis BioDyne, Tartu, Estonia), 0.7 µl 50 mM MgCl2 (Solis BioDyne), 0.75 µl 10 mM deoxyribonucleotide triphosphates (Solis BioDyne), 0.3 µl 100 µM forward primer and 0.3 µl of 100 µM reverse primer (primer sequences are listed in Table I) (Integrated DNA Technologies, Coralville, IA, USA) and 0.5 µl 5 U/µl Hot Start DNA Polymerase (Solis BioDyne). Thermocycling consisted of one cycle of enzyme activation (at 95°C for 15 min), followed by 35 cycles of DNA amplification (at 95°C for 45 sec, at 59°C for 45 sec and at 72°C for 1 min). PCR products were then separated by agarose gel electrophoresis (1.5% agarose gel in tris-borate-EDTA; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and purified with Invisorb spin columns (Invitek, Inc., Hayward, CA, USA). PCR-purified products were re-amplified with terminating nucleotides using Big Dye Terminator v3.1 (Applied Biosystems; Thermo Fisher Scientific, Inc.). Sequencing analysis was performed with an ABI Prism 3100 Avant automated sequencer (Thermo Fisher Scientific, Inc.) equipped with 36 cm capillary array filled with POP6 polymer (Thermo Fisher Scientific, Inc.). Electropherograms were analyzed using Sequencing Analysis software (Applied Biosystems, version 5.1; Thermo Fisher Scientific, Inc.).

Table I.

Primer sequences.

Table I.

Primer sequences.

PrimerSequence (5′-3′)
RS1_exon 1F GGAAAGCCATCCACACAAAG
RS1_exon 1R GGTTAACTTGATGGGGCTCA
RS1_exon 2F TCCTGACCTCAAGTGATCTGC
RS1_exon 2R TTCTTCCAGAAGGGGTGTTG
RS1_exon 3F GGAGAAAACCCGCATTAACA
RS1_exon 3R GACGATGCATAAGGACTGAGTG
RS1_exon 4F CCACCACGCCAGTTAATTTT
RS1_exon 4R GCAAAGCAGATGGGTTTGTT
RS1_exon 5F ACAGAGGGCAGTGACAGGAG
RS1_exon 5R GGAGACAAGGCTCAGACTGC
RS1_exon 6F ACCCAGCACTGCAGTTACAA
RS1_exon 6R GGGCTAGCTCCAGAAAGGAA

[i] F, forward; R, reverse.

Results

Patients

Three members of a family with two brothers affected by XLRS were studied. The first proband was a 41-year-old male and complained of mild visual disturbance since the age of 9, where medical records reported a spoke-wheel appearance of the macula and a BCVA of 0.8. His medical history was unremarkable. At the first presentation, BCVA was 0.4 in oculus uterque and remained stable for all the follow-up (2007 through to 2016). Fundus examination reported the typical spoke wheel appearance of the macula in both eyes. In the right eye, a peripheral schisis in the inferior-temporal retina reaching the vascular arcade was also observed. Macular abnormalities were best recognized with FAF that indicated hyper- and hypo-FAF arranged in a radial pattern. Fluorescein angiography did not reveal any late leakage and confirmed the non-exudative origin of macular cysts.

On first examination with OCT, macular thickness was significantly increased. OCT scans presented a cystic degeneration, primarily involving the INL, though some small cysts were detected in the outer plexiform layer (OPL) and in the ganglion cell layer (GCL). In RE, peripheral scans documented retinal schisis extending inferiorly. The first OCT was acquired when the patient was ~31 years old and BCVA was already reduced. In the right eye, the ten-year follow-up demonstrated that there were no noticeable variations in retinal morphology or in the overall width of the peripheral schisis. However, the progressive straining of retinal layers breaks up the wall of macular cysts, weakening the retinal structure. Indeed, in the left eye, there was a moderate reduction in central retinal thickness (from 448 µm to 402 µm) that precedes the late, atrophic stage of XLRS. Outer retinal layers (external limiting membrane, inner segment/outer segment junction and photoreceptor layer) demonstrated diffuse atrophic changes with no significant progression during the follow-up (Fig. 1). Goldmann perimetry indicated in RE an absolute scotoma superiorly, that matched the peripheral retinal schisis. The dark-adapted 0.01 ERG (rod response) was reduced. The dark-adapted 3.0 ERG (combined rod-cone response) showed a b/a ratio <1, which matched the definition of an electronegative ERG. The patient reported a delay in implicit time and a decrease in the amplitude of the b-wave in the light adapted 3.0 ERG. A delayed light adapted 30 Hz flicker peak time and decreased amplitude was also present. The diagnosis of XLRS was proposed and all family members were then invited for eye examination. His brother was a 30-year-old male. The diagnosis of unspecified retinal dystrophy was made when he was 8 years old and he presented with a initial visual loss associated with characteristic spoke wheel appearance of the macula. At the examination, BCVA was 0.5 in RE and 0.6 in LE and progressively decreased to 0.4 in RE and 0.3 in LE during follow-up. Fundus examination, OCT and retinal angiography revealed the typical features of XLRS. An electronegative ERG further supported the diagnosis. First OCT scans presented irregular empty spaces in the INL. Some small cysts could be observed in the GCL. Atrophic changes involving outer retinal layers (external limiting membrane, inner segment/outer segment junction and photoreceptor layer) were recognizable. During the follow-up, the OCT scans revealed a progressive enlargement and coalescence of the cysts located in the INL, with subsequent retinal thickening. Small cysts in the GCL did not show further enlargement. Atrophic changes of outer retinal layers remained stable during follow-up (Fig. 2).

Ophthalmologic examination of their sister (21 years old) and of their father (69 years old) was normal. Their mother was deceased, but no significant visual disturbances were reported, even though it was not possible to ascertain if she was a healthy carrier or if she developed a de novo mutation. Indeed, ocular diseases in their maternal grandfather were not reported. Then, the authors drew a genealogical tree based on available information (Fig. 3).

Genetic testing

Screening of RS1 by sequencing of PCR-amplified DNA identified a novel missense mutation in exon 6 in both XLRS patients. This mutation from A to T at nucleotide position 635 changes the nonpolar isoleucine to positively charged asparagine (p.Ile212Asn).

This substitution was predicted to be harmful to protein function by in silico analysis (Polyphen2=0.989; Fig. 3).

Discussion

Mutations in the RS1 gene are responsible for inherited and sporadic XLRS. To date 191 causative genetic variations have been identified. Among all described variations, missense mutations represent the most recurrent subtype (100 mutations of 191). Mutations can affect all regions of the RS gene, although a substantial clustering is observed within the region coding for the discoidin domain (85 of 191) (4). The novel missense mutation that we found in the patients is capable to produce the typical clinical phenotype of XLRS, as it affects the discoidin domain of retinoschisin.

Retinoschisin is a 24-kDa protein expressed exclusively by photoreceptors and bipolar cells in the retina and pineal gland. RS1 is secreted as a soluble disulfide bond-stabilized octamer. Most of the monomer (157 amino acids) comprises a discoidin domain, a globular fold that is highly conserved in a family of extracellular or transmembrane proteins implicated in cell adhesion or cell-cell interactions. The high number of mutations within the discoidin domain indicates that it is essential for the normal function of this protein. Although the role of retinoschisin in the retina is not well understood, it has been hypothesized that it works as a cell adhesion protein to maintain the structural organization of the retina and of the photoreceptor-bipolar synapse (4,5,10,12).

Differential diagnosis of XLRS comprises cystic changes of the macula that could arise from exudative and degenerative disorders or abnormalities of the vitreoretinal interface. Degenerative cystoid maculopathies may be hereditary [XLRS, Enhanced S-Cone/Goldmann Favre Syndrome (ESCS/GFS)] or acquired [microcystic macular edema (MME)]. All these conditions do not show late leakage on fluorescein angiography.

ESCS/GFS is a recessive disorder caused by mutations in the NR2E3 gene. This gene encodes for a nuclear receptor expressed in the outer nuclear layer of the retina. It suppresses cone differentiation during embryogenesis, therefore loss of NR2E3 results in retinas with a decreased number of rod photoreceptors and an increase in cones, predominantly expressing the S-cone opsin (13). Time trend of macular cysts development in ESCS/GFS resemble what happens in XLRS. The progressive enlargement and coalescence of macular cysts in young adulthood is followed by resolution of the schisis and reduction of macular thickness (14). However, location of cystic spaces seems different from XLRS. The analysis of published images shows large confluent cysts in the outer retinal layer associated with small and well-demarcated cysts in the INL (15,16).

MME is a recently described OCT entity, characterized by the appearance of small retinal cysts in patients with optic neuritis and optic atrophy of various aetiology (multiple sclerosis, neuromielitis optica, glaucoma) probably due to retrograde synaptic degeneration. As in XLRS, MME cysts involve the INL, however they do not seem to enlarge significantly with time. Moreover in MME cysts are usually circumscribed to the parafoveal region (17,18), whereas in XLRS cystic degeneration may extend beyond the vascular arcades.

Diagnosis of XLRS in the patients of the present study was strongly supported by clinical and instrumental findings. Indeed both patients presented with the classical phenotype of XLRS, with spoke-wheel maculopathy, association with peripheral schisis and ERG b-wave suppression. Ophthalmological history, regarding age of onset of symptoms and visual decline follows the typical course described in literature (7). Visual symptoms begin in the first decade, and then visual function progressively decreases, remaining stable in the following 10 years.

SD-OCT images collected in the two patients documented the natural history of the disease, characterized by the formation of small cysts located in the INL that progressively enlarge and coalesce. Gradually, the stretching of the wall of macular cysts weakens the retinal structure leading to the collapse of the cysts. Small isolated cysts were also observed in the GCL and OPL that, differently from those located in the INL, do not seem to increase over time. The possible presence of small cysts not determining macular splitting have been previously described by Gregori et al (19), who also found similar empty spaces in the ONL and in the GCL. The same paper reported that macular schisis may occur also in the OPL (19). An immunochemistry study (20) in the normal mouse eye detected retinoschisin protein in all retinal layers; this may explain why retinal schisis can develop in different layers, even if some layers (GCL and RNFL) seem relatively resistant. The foveomacular retinoschisis is usually located in the INL, while the extramacular schisis could be equally found in the INL, ONL and GCL/NFL (21). In the present study, cystic degeneration at the level of peripheral schisis in RE of the first proband was located in the INL (Fig. 1).

Since clinical and instrumental findings were highly suggestive for XLRS, the authors performed genetic analysis. The sequencing of the proband DNA, revealed the hemizygous missense mutation p.lle212Asn (c.635A>T) in the RS1 gene. The same mutation was identified in the proband's brother and has never been reported before. It consists in a substitution of the non-polar amino acid, isoleucine, with the polar amino acid, asparagine. The mutation is located in a domain in which several genetic alterations associated with XLRS has been found (positions p.206, p.209, p.211, p.213, p.2015 and p.219). Furthermore, the PolyPhen-2 predictor has given to this mutation a high likelihood of protein dysfunction.

Given the high number of RS1 mutations, several studies investigated a possible correlation between genotype and phenotype. Even though some reports describe a correlation between specific variations and the severity of clinical phenotype (22,23), the majority of published studies found that XLRS patients had relatively uniform clinical manifestations, although with great intra-familial variation in age at onset and progression (1,2426).

Interestingly, the deletion of exon 1 and promoter region, which causes complete absence of retinoschisin, is associated with a wide variability of the phenotype, suggesting that other genetic and/or epigenetic factors are likely to act as significant phenotypic modifiers in XLRS (27,28).

The absence of leakage on fluorescein angiography in XLRS patients suggests that vascular hyperpermeability serves a minor role, if any, in the pathogenesis of the disease. However, both oral and topical carbonic anhydrase inhibitors (CAIs) have been used successfully in the management of XLRS. CAIs act both on retinal and RPE cell function by acidifying the subretinal space, decreasing the standing potential as well as raising retinal adhesiveness, probably by increasing RPE fluid transport (29).

A promising approach for XLRS treatment is gene therapy. The disease is an excellent candidate for gene therapy as the majority of mutations have been shown to lead to a complete deficiency of the secreted protein in the retinal structures. Genetic treatment studies, conducted in rabbits and mouse, with intravitreal administration of adeno-associated viral vector coding the human RS1 gene demonstrated an improvement in retinal structure and function (30,31). Other strategies comprise the intravitreal administration of adipose-derived mesenchymal stem cells, genetically modified to secrete the human RS1 gene, or solid lipid nanoparticles inducing the expression of retinoschisin in photoreceptors. Both treatments demonstrated morphological and functional improvements in mouse models (32,33). Two phase I/II trials are testing safety and efficacy of adeno-associated viral vectors, called AAV8-scRS/IRBPhRS and rAAV2tYF-CB-hRS1, as a gene therapy approach in XLRS patients. Viral vectors have been demonstrated to be able to shuttle normal RS1 DNA into retinal cells. Results are expected from 2017 (34,35).

The present study describes long-term morphological and functional changes of XLRS patients affected by a novel RS1 mutation. Although the correspondence between genotype and phenotype is still under debate, is reasonable that siblings affected by XLRS could share other genetic and/or epigenetic factors capable to influence clinical course of the disease and perhaps treatment response to upcoming genetic therapies.

References

1 

The Retinoschisis Consortium: Functional implications of the spectrum of mutations found in 234 cases with X-linked juvenile retinoschisis. Hum Mol Genet. 7:1185–1192. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Sauer CG, Gehrig A, Warneke-Wittstock R, Marquardt A, Ewing CC, Gibson A, Lorenz B, Jurklies B and Weber BH: Positional cloning of the gene associated with X-linked juvenile retinoschisis. Nat Genet. 17:164–170. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Mooy CM, Van Den Born LI, Baarsma S, Paridaens DA, Kraaijenbrink T, Bergen A and Weber BH: Hereditary X-linked juvenile retinoschisis: A review of the role of Müller cells. Arch Ophthalmol. 120:979–984. 2002.PubMed/NCBI

4 

Molday RS, Kellner U and Weber BH: X-linked juvenile retinoschisis: Clinical diagnosis, genetic analysis, and molecular mechanisms. Prog Retin Eye Res. 31:195–212. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Tantri A, Vrabec TR, Cu-Unjieng A, Frost A, Annesley WH Jr and Donoso LA: X-linked retinoschisis: A clinical and molecular genetic review. Surv Ophthalmol. 49:214–230. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Sikkink SK, Biswas S, Parry NRA, Stanga PE and Trump D: X-linked retinoschisis: An update. J Med Genet. 44:225–232. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Renner AB, Kellner U, Fiebig B, Cropp E, Foerster MH and Weber BH: ERG variability in X-linked congenital retinoschisis patients with mutations in the RS1 gene and the diagnostic importance of fundus autofluorescence and OCT. Doc Ophthalmol. 116:97–109. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Yu J, Ni Y, Keane PA, Jiang C, Wang W and Xu G: Foveomacular schisis in juvenile X-linked retinoschisis: An optical coherence tomography study. Am J Ophthalmol. 149:973–978.e2. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Wang T, Waters CT, Rothman AMK, Jakins TJ, Römisch K and Trump D: Intracellular retention of mutant retinoschisin is the pathological mechanism underlying X-linked retinoschisis. Hum Mol Genet. 11:3097–3105. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Wu WW, Wong JP, Kast J and Molday RS: RS1, a discoidin domain-containing retinal cell adhesion protein associated with X-linked retinoschisis, exists as a novel disulfide-linked octamer. J Biol Chem. 280:10721–10730. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Molday LL, Hicks D, Sauer CG, Weber BHF and Molday RS: Expression of X-linked retinoschisis protein RS1 in photoreceptor and bipolar cells. Invest Ophthalmol Vis Sci. 42:816–825. 2001.PubMed/NCBI

12 

Tolun G, Vijayasarathy C, Huang R, Zeng Y, Li Y, Steven AC, Sieving PA and Heymann JB: Paired octamer rings of retinoschisin suggest a junctional model for cell-cell adhesion in the retina. Proc Natl Acad Sci USA. 113:5287–5292. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Yzer S, Barbazetto I, Allikmets R, van Schooneveld MJ, Bergen A, Tsang SH, Jacobson SG and Yannuzzi LA: Expanded clinical spectrum of enhanced S-cone syndrome. JAMA Ophthalmol. 131:1324–1330. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Sohn EH, Chen FK, Rubin GS, Moore AT, Webster AR and MacLaren RE: Macular function assessed by microperimetry in patients with enhanced S-cone syndrome. Ophthalmology. 117:1199–1206.e1. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Bušić M, Bjeloš M, Bosnar D, Ramić S and Bušić I: Cystoid macular lesions are resistant to topical dorzolamide treatment in enhanced S-cone syndrome child. Doc Ophthalmol. 132:67–73. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Salvatore S, Fishman GA and Genead MA: Treatment of cystic macular lesions in hereditary retinal dystrophies. Surv Ophthalmol. 58:560–584. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Sigler EJ: Microcysts in the inner nuclear layer, a nonspecific SD-OCT sign of cystoid macular edema. Invest Ophthalmol Vis Sci. 55:3282–3284. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Murata N, Togano T, Miyamoto D, Ochiai S and Fukuchi T: Clinical evaluation of microcystic macular edema in patients with glaucoma. Eye (Lond). 30:1502–1508. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Gregori NZ, Berrocal AM, Gregori G, Murray TG, Knighton RW, Flynn HW Jr, Dubovy S, Puliafito CA and Rosenfeld PJ: Macular spectral-domain optical coherence tomography in patients with X linked retinoschisis. Br J Ophthalmol. 93:373–378. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Prenner JL, Capone A Jr, Ciaccia S, Takada Y, Sieving PA and Trese MT: Congenital X-linked retinoschisis classification system. Retina. 26 Suppl:S61–S64. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Gregori NZ, Lam BL, Gregori G, Ranganathan S, Stone EM, Morante A, Abukhalil F and Aroucha PR: Wide-field spectral-domain optical coherence tomography in patients and carriers of X-linked retinoschisis. Ophthalmology. 120:169–174. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Atchaneeyasakul LO, Trinavarat A, Pituksung A, Jinda W, Thongnoppakhun W and Limwongse C: Mutations in the XLRS1 gene in Thai families with X-linked juvenile retinoschisis. Jpn J Ophthalmol. 54:89–93. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Li X, Ma X and Tao Y: Clinical features of X linked juvenile retinoschisis in Chinese families associated with novel mutations in the RS1 gene. Mol Vis. 13:804–812. 2007.PubMed/NCBI

24 

Kim SY, Ko HS, Yu YS, Hwang JM, Lee JJ, Kim SY, Kim JY, Seong MW, Park KH and Park SS: Molecular genetic characteristics of X-linked retinoschisis in Koreans. Mol Vis. 15:833–843. 2009.PubMed/NCBI

25 

Lesch B, Szabó V, Kánya M, Somfai GM, Vámos R, Varsányi B, Pámer Z, Knézy K, Salacz G, Janáky M, et al: Clinical and genetic findings in Hungarian patients with X-linked juvenile retinoschisis. Mol Vis. 14:2321–2332. 2008.PubMed/NCBI

26 

Riveiro-Alvarez R, Trujillo-Tiebas MJ, Gimenez-Pardo A, Garcia-Hoyos M, Lopez-Martinez MA, Aguirre-Lamban J, Garcia-Sandoval B, Vazquez-Fernandez del Pozo S, Cantalapiedra D, Avila-Fernandez A, et al: Correlation of genetic and clinical findings in Spanish patients with X-linked juvenile retinoschisis. Invest Ophthalmol Vis Sci. 50:4342–4350. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Eksandh LC, Ponjavic V, Ayyagari R, Bingham EL, Hiriyanna KT, Andréasson S, Ehinger B and Sieving PA: Phenotypic expression of juvenile X-linked retinoschisis in Swedish families with different mutations in the XLRS1 gene. Arch Ophthalmol. 118:1098–1104. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Iannaccone A, Mura M, Dyka FM, Ciccarelli ML, Yashar BM, Ayyagari R, Jablonski MM and Molday RS: An unusual X-linked retinoschisis phenotype and biochemical characterization of the W112C RS1 mutation. Vision Res. 46:3845–3852. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Verbakel SK, van de Ven JP, Le Blanc LMP, Groenewoud JMM, de Jong EK, Klevering BJ and Hoyng CB: Carbonic anhydrase inhibitors for the treatment of cystic macular lesions in children with X-linked juvenile retinoschisis. Invest Ophthalmol Vis Sci. 57:5143–5147. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Marangoni D, Bush RA, Zeng Y, Wei LL, Ziccardi L, Vijayasarathy C, Bartoe JT, Palyada K, Santos M, Hiriyanna S, et al: Ocular and systemic safety of a recombinant AAV8 vector for X-linked retinoschisis gene therapy: GLP studies in rabbits and Rs1-KO mice. Mol Ther Methods Clin Dev. 5:160112016. View Article : Google Scholar : PubMed/NCBI

31 

Zeng Y, Petralia RS, Vijayasarathy C, Wu Z, Hiriyanna S, Song H, Wang YX, Sieving PA and Bush RA: Retinal structure and gene therapy outcome in retinoschisin-deficient mice assessed by spectral-domain optical coherence tomography. Invest Ophthalmol Vis Sci. 57:OCT277–OCT287. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Bashar AE, Metcalfe AL, Viringipurampeer IA, Yanai A, Gregory-Evans CY and Gregory-Evans K: An ex vivo gene therapy approach in X-linked retinoschisis. Mol Vis. 22:718–733. 2016.PubMed/NCBI

33 

Apaolaza PS, Del Pozo-Rodríguez A, Solinís MA, Rodríguez JM, Friedrich U, Torrecilla J, Weber BH and Rodríguez-Gascón A: Structural recovery of the retina in a retinoschisin-deficient mouse after gene replacement therapy by solid lipid nanoparticles. Biomaterials. 90:40–49. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Study of RS1 ocular gene transfer for X-linked retinoschisis. https://www.clinicaltrials.govDecember. 2016

35 

Safety and efficacy of rAAV-hRS1 in patients with X-linked retinoschisis (XLRS). https://www.clinicaltrials.govDecember. 2016

Related Articles

Journal Cover

September-2017
Volume 7 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Piermarocchi S, Miotto S, Colavito D, Del Giudice E, Leon A, Maritan V, Piermarocchi R and Tormene AP: Long-term rearrangement of retinal structures in a novel mutation of X-linked retinoschisis. Biomed Rep 7: 241-246, 2017.
APA
Piermarocchi, S., Miotto, S., Colavito, D., Del Giudice, E., Leon, A., Maritan, V. ... Tormene, A.P. (2017). Long-term rearrangement of retinal structures in a novel mutation of X-linked retinoschisis. Biomedical Reports, 7, 241-246. https://doi.org/10.3892/br.2017.954
MLA
Piermarocchi, S., Miotto, S., Colavito, D., Del Giudice, E., Leon, A., Maritan, V., Piermarocchi, R., Tormene, A. P."Long-term rearrangement of retinal structures in a novel mutation of X-linked retinoschisis". Biomedical Reports 7.3 (2017): 241-246.
Chicago
Piermarocchi, S., Miotto, S., Colavito, D., Del Giudice, E., Leon, A., Maritan, V., Piermarocchi, R., Tormene, A. P."Long-term rearrangement of retinal structures in a novel mutation of X-linked retinoschisis". Biomedical Reports 7, no. 3 (2017): 241-246. https://doi.org/10.3892/br.2017.954