Association analysis of FAS‑670A/G and FASL‑844C/T polymorphisms with risk of generalized aggressive periodontitis disease

Asgari,Rezvan; Yari,Kheirollah; Mansouri,Kamran; Bakhtiari,Mitra

doi:10.3892/br.2018.1060

Association analysis of FAS‑670A/G and FASL‑844C/T polymorphisms with risk of generalized aggressive periodontitis disease

Authors:
- Rezvan Asgari
- Kheirollah Yari
- Kamran Mansouri
- Mitra Bakhtiari
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Affiliations: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah 67148‑69914, Iran, Department of Anatomical Sciences and Cell Biology, Kermanshah University of Medical Sciences, Kermanshah 67148‑69914, Iran
Published online on: February 8, 2018 https://doi.org/10.3892/br.2018.1060
Pages: 391-395

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Abstract

The interaction of FAS/FAS ligand (FASL) serves an important role in the upregulation of apoptotic processes through different mechanisms in cells. Previous studies have established that the polymorphisms FAS‑670A/G and FASL‑844C/T are associated with risk of generalized aggressive periodontitis (GAP) in different ethnic populations. Therefore, in the present study, it was investigated for the first time whether FAS‑670A/G and FASL‑844C/T polymorphisms were associated with risk of GAP in Iran. This case‑control study performed the polymerase chain reaction‑restriction fragment length polymorphism method in 25 patients with GAP and 110 normal subjects as controls. The results indicated that there was no significant difference in FAS‑670A/G genotype frequency between the GAP and control groups. A higher frequency of the combined genotype (AG+GG) was observed in the GAP patients (96.0%) compared with the control subjects (90.9%), though this was not significant [χ2=0.705, degrees of freedom (df)=1, P=0.401]. Similarly, the prevalence of the G allele was non‑significantly higher in the GAP group (62.0%) compared with that in the controls (60.0%; χ2=0.012, df=1, P=0.913). For FASL‑844C/T polymorphism, the frequency of the combined genotype (CT+TT) was higher in the GAP group (96.0%) when compared with the control subjects (91.8%); however its association was not statistically significant (χ2=0.519, df=1, P=0.471). The frequency of the T allele only marginally differed between the groups, being 60.0% in the GAP group and 50.9% in the controls (χ2=3.627, df=1, P=0.057). These results indicated that there were no significant associations between the FAS‑670A/G and FASL‑844C/T polymorphisms and the risk of disease in GAP patients when compared with normal individuals.

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