Sinomenine hydrochloride inhibits cell survival in human hepatoma Huh7 cells

Wang,Ying; Li,Ming; Yu,Xuesong; Chen,Ali; Ding,Ying; Wang,Yan; Wang,Yan

doi:10.3892/br.2018.1084

Sinomenine hydrochloride inhibits cell survival in human hepatoma Huh7 cells

Authors:
- Ying Wang
- Ming Li
- Xuesong Yu
- Ali Chen
- Ying Ding
- Yan Wang
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Affiliations: Experimental Center, School of Chemistry Engineering, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Pathology and Pathophysiology, School of Basic Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Marine Pharmacy, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Chinese Pharmaceutical Manufacturing, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China
Published online on: April 2, 2018 https://doi.org/10.3892/br.2018.1084
Pages: 510-516
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the effect of sinomenine hydrochloride (SIN) on cell survival/proliferation in the human hepatoma cell line Huh7, as well as determine the underlying mechanisms. Three different doses of SIN, 140, 280 and 560 µM, were tested. Cellular apoptosis and cell cycle distribution were analyzed by flow cytometry. Western blotting was used to determine protein levels of the apoptosis‑associated regulators, cleaved caspase 3, B‑cell lymphoma‑2 (Bcl‑2)‑associated X protein (Bax), Bcl‑2 homologous antagonist/killer (Bak) and Bcl‑extra large (Bcl‑xl), as well as the cell cycle‑related regulators, p21 and p27. It was observed that the three doses of SIN were able to suppress Huh7 cell survival/proliferation, and efficiently induce cellular apoptosis as well as multiphase cell cycle arrest. Mechanistically, SIN treatment upregulated the levels of the pro‑apoptotic regulators, cleaved caspase 3 and Bax, and downregulated the level of anti‑apoptotic Bcl‑xl. Additionally, SIN treatment also increased the protein levels of p21 and p27, as two regulators functioning to slow cell cycle progression. Taken together, the present studied indicated SIN to be a promising compound for the treatment of hepatocellular carcinoma, based on its apparent effect in modulating cell apoptosis and the cell cycle in Huh7 cells in vitro.

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