Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia

Zhu,Guangbin; Xie,Changying; Yang,Zhonghua; Wang,Yongzhi; Chen,Dong; Wang,Xinghuan

doi:10.3892/br.2018.1089

Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia

Authors:
- Guangbin Zhu
- Changying Xie
- Zhonghua Yang
- Yongzhi Wang
- Dong Chen
- Xinghuan Wang
View Affiliations

Affiliations: Department of Urology, Daqing Oilfield General Hospital, Daqing, Heilongjiang 163000, P.R. China, Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: April 23, 2018 https://doi.org/10.3892/br.2018.1089
Pages: 529-534

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study aimed to determine whether the expression of transient receptor potential channel 5 (TRPC5) protein is altered in spermatozoa of patients with varicocele‑associated asthenozoospermia. TRPC5 expression in spermatozoa was determined by polymerase chain reaction and western blotting analyses, and indirect immunofluorescence was used for identification and immunolocalization of the TRPC5 channel in human sperm. Sperm motility and superoxide dismutase (SOD) activity were also determined with a computer‑assisted semen analysis system and assay kit, respectively. Compared with levels in control subjects, it was identified that TRPC5 protein expression, SOD activity and cellular motility in the sperm of patients with varicocele‑associated asthenozoospermia were reduced (P<0.001). Furthermore, the expression of TRPC5 was positively correlated with sperm motility (r=0.781, P<0.001) and SOD activity (r=0.933, P<0.001), indicated by partial correlation analysis. The present study may provide a novel target for the study and treatment of varicocele‑associated asthenozoospermia.

View Figures

View References

1	Darszon A, Labarca P, Nishigaki T and Espinosa F: Ion channels in sperm physiology. Physiol Rev. 79:481–510. 1999. View Article : Google Scholar : PubMed/NCBI
2	Kumar PG and Shoeb M: The role of trp ion channels in testicular function. Adv Exp Med Biol. 704:881–908. 2011. View Article : Google Scholar : PubMed/NCBI
3	Cox T and Peterson RN: Identification of calcium conducting channels in isolated boar sperm plasma membranes. Biochem Biophys Res Commun. 161:162–168. 1989. View Article : Google Scholar : PubMed/NCBI
4	Nilius B: TRP channels in disease. Biochim Biophys Acta. 1772:805–812. 2007. View Article : Google Scholar : PubMed/NCBI
5	Voets T, Talavera K, Owsianik G and Nilius B: Sensing with TRP channels. Nat Chem Biol. 1:85–92. 2005. View Article : Google Scholar : PubMed/NCBI
6	Wes PD, Chevesich J, Jeromin A, Rosenberg C, Stetten G and Montell C: TRPC1, a human homolog of a Drosophila store-operated channel. Proc Natl Acad Sci USA. 92:9652–9656. 1995. View Article : Google Scholar : PubMed/NCBI
7	Zhu X, Jiang M, Peyton M, Boulay G, Hurst R, Stefani E and Birnbaumer L: trp, a novel mammalian gene family essential for agonist-activated capacitative Ca²⁺ entry. Cell. 85:661–671. 1996. View Article : Google Scholar : PubMed/NCBI
8	Clapham DE: TRP channels as cellular sensors. Nature. 426:517–524. 2003. View Article : Google Scholar : PubMed/NCBI
9	Montell C: Physiology, phylogeny, and functions of the TRP superfamily of cation channels. Sci STKE. 2001:re12001.PubMed/NCBI
10	Putney JW Jr: The enigmatic TRPCs: Multifunctional cation channels. Trends Cell Biol. 14:282–286. 2004. View Article : Google Scholar : PubMed/NCBI
11	Sutton KA, Jungnickel MK, Wang Y, Cullen K, Lambert S and Florman HM: Enkurin is a novel calmodulin and TRPC channel binding protein in sperm. Dev Biol. 274:426–435. 2004. View Article : Google Scholar : PubMed/NCBI
12	Treviño CL, Serrano CJ, Beltrán C, Felix R and Darszon A: Identification of mouse trp homologs and lipid rafts from spermatogenic cells and sperm. FEBS Lett. 509:119–125. 2001. View Article : Google Scholar : PubMed/NCBI
13	Castellano LE, Treviño CL, Rodríguez D, Serrano CJ, Pacheco J, Tsutsumi V, Felix R and Darszon A: Transient receptor potential (TRPC) channels in human sperm: Expression, cellular localization and involvement in the regulation of flagellar motility. FEBS Lett. 541:69–74. 2003. View Article : Google Scholar : PubMed/NCBI
14	World Health Organization, . WHO Manual for the Standardised Investigation and Diagnosis of Infertile Couple. Cambridge University Press; Cambridge, UK: pp. 102000
15	Dunson DB, Baird DD and Colombo B: Increased infertility with age in men and women. Obstet Gynecol. 103:51–56. 2004. View Article : Google Scholar : PubMed/NCBI
16	Sharlip ID, Jarow JP, Belker AM, Lipshultz LI, Sigman M, Thomas AJ, Schlegel PN, Howards SS, Nehra A, Damewood MD, et al: Best practice policies for male infertility. Fertil Steril. 77:873–882. 2002. View Article : Google Scholar : PubMed/NCBI
17	Kamischke A and Nieschlag E: Varicocele treatment in the light of evidence-based andrology. Hum Reprod Update. 7:65–69. 2001. View Article : Google Scholar : PubMed/NCBI
18	World Health Organization, . Laboratory Manual for the Examination and Processing of Human Semen and Sperm-Cervical Mucus Interaction. 5th edition. Cambridge University Press; Cambridge, UK: pp. 8–9. 2010
19	Parrish JJ, Krogenaes A and Susko-Parrish JL: Effect of bovine sperm separation by either swim-up or Percoll method on success of in vitro fertilization and early embryonic development. Theriogenology. 44:859–869. 1995. View Article : Google Scholar : PubMed/NCBI
20	He DX, Gu XT, Jiang L, Jin J and Ma X: A methylation-based regulatory network for microRNA 320a in chemoresistant breast cancer. Mol Pharmacol. 86:536–547. 2014. View Article : Google Scholar : PubMed/NCBI
21	Darszon A, Beltrán C, Felix R, Nishigaki T and Treviño CL: Ion transport in sperm signaling. Dev Biol. 240:1–14. 2001. View Article : Google Scholar : PubMed/NCBI
22	Ho HC and Suarez SS: Hyperactivation of mammalian spermatozoa: Function and regulation. Reproduction. 122:519–526. 2001. View Article : Google Scholar : PubMed/NCBI
23	Okada T, Shimizu S, Wakamori M, Maeda A, Kurosaki T, Takada N, Imoto K and Mori Y: Molecular cloning and functional characterization of a novel receptor-activated TRP Ca²⁺ channel from mouse brain. J Biol Chem. 273:10279–10287. 1998. View Article : Google Scholar : PubMed/NCBI
24	French DB, Desai NR and Agarwal A: Varicocele repair: Does it still have a role in infertility treatment? Curr Opin Obstet Gynecol. 20:269–274. 2008. View Article : Google Scholar : PubMed/NCBI
25	Naughton CK, Nangia AK and Agarwal A: Pathophysiology of varicoceles in male infertility. Hum Reprod Update. 7:473–481. 2001. View Article : Google Scholar : PubMed/NCBI
26	Marmar JL: The pathophysiology of varicoceles in the light of current molecular and genetic information. Hum Reprod Update. 7:461–472. 2001. View Article : Google Scholar : PubMed/NCBI
27	Makker K, Agarwal A and Sharma R: Oxidative stress & male infertility. Indian J Med Res. 129:357–367. 2009.PubMed/NCBI
28	Armstrong JS, Rajasekaran M, Chamulitrat W, Gatti P, Hellstrom WJ and Sikka SC: Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Radic Biol Med. 26:869–880. 1999. View Article : Google Scholar : PubMed/NCBI