Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats

  • Authors:
    • Saman Sargazi
    • Hamidreza Galavi
    • Sadegh Zarei
  • View Affiliations

  • Published online on: April 23, 2018     https://doi.org/10.3892/br.2018.1090
  • Pages: 565-570
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Diazinon is an organophosphate toxicant that has been identified to induce oxidative stress within biological systems through altering biochemical and hematological indices, by generating free radicals while deteriorating the cellular antioxidant defense system. The present study was designed to evaluate the effectiveness of tert‑butylhydroquinone (TBHQ), a synthetic food additive, in serving as an antioxidant against sub‑lethal diazinon‑induced oxidative stress in male Wistar rats. Animals were randomly divided into 4 groups: Control (treated with corn oil as a vehicle), diazinon (10 mg/kg/day), TBHQ (0.028 g/kg of diet) and a combination group of TBHQ (0.028 g/kg of diet) plus diazinon (10 mg/kg/day). All animals were treated orally once a day by gastric tube and treatments were continued for 7 weeks. Hematological indices, including red blood cell (RBC) indices and white blood cell (WBC) and platelet counts, were measured at the end of the treatment schedule. On comparison of the treatment groups with the untreated control group, RBC count, hemoglobin (Hb), hematocrit (Hct), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were significantly decreased in the diazinon and TBHQ+diazinon groups, while MCHC in the TBHQ group and MCV in the diazinon and TBHQ+diazinon groups were significantly increased. When the diazinon and TBHQ+diazinon groups were compared with the TBHQ group, RBC, Hb, Hct, and MCHC were significantly decreased whereas MCV was increased in both groups. Additionally, when comparing the TBHQ+diazinon group with the diazinon group, MCHC was determined to be significantly decreased. The results of the current experiments suggested that TBHQ could not efficiently protect blood cells against diazinon toxicity.
View Figures
View References

Related Articles

Journal Cover

June-2018
Volume 8 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sargazi S, Galavi H and Zarei S: Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats. Biomed Rep 8: 565-570, 2018
APA
Sargazi, S., Galavi, H., & Zarei, S. (2018). Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats. Biomedical Reports, 8, 565-570. https://doi.org/10.3892/br.2018.1090
MLA
Sargazi, S., Galavi, H., Zarei, S."Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats". Biomedical Reports 8.6 (2018): 565-570.
Chicago
Sargazi, S., Galavi, H., Zarei, S."Evaluation of attenuative effect of tert‑butylhydroquinone against diazinon‑induced oxidative stress on hematological indices in male Wistar rats". Biomedical Reports 8, no. 6 (2018): 565-570. https://doi.org/10.3892/br.2018.1090