Effect of progesterone on nitric oxide/cyclic guanosine monophosphate signaling and contraction in gastric smooth muscle cells Corrigendum in /10.3892/br.2019.1251

Al‑Shboul,Othman  A.; Mustafa,Ayman  G.; Omar,Amal   Abu; Al‑Dwairi,Ahmed  N.; Alqudah,Mohammad  A.; Nazzal,Mona  S.; Alfaqih,Mahmoud  A.; Al‑Hader,Rami  A.

doi:10.3892/br.2018.1161

Effect of progesterone on nitric oxide/cyclic guanosine monophosphate signaling and contraction in gastric smooth muscle cells

Corrigendum in 10.3892/br.2019.1251

Authors:
- Othman A. Al‑Shboul
- Ayman G. Mustafa
- Amal Abu Omar
- Ahmed N. Al‑Dwairi
- Mohammad A. Alqudah
- Mona S. Nazzal
- Mahmoud A. Alfaqih
- Rami A. Al‑Hader
View Affiliations

Affiliations: Department of Physiology and Biochemistry, Faculty of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan, Department of Anatomy, Faculty of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan, Department of Physiology and Biochemistry, Princess Basma Teaching Hospital, Faculty of Medicine, Jordan University of Science and Technology, Irbid 21110, Jordan
Published online on: October 18, 2018 https://doi.org/10.3892/br.2018.1161
Pages: 511-516

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Abstract

Previous studies have shown that progesterone could inhibit muscle contraction in various sites of the gastrointestinal tract. The underlying mechanisms responsible for these inhibitory effects of progesterone are not fully known. The aim of the current study was to investigate the effect of progesterone on the nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) pathway and muscle contraction in the stomach. Single gastric smooth muscle cells from female Sprague‑Dawley rats were used. The expression of progesterone receptor (PR) mRNA was analyzed by reverse transcription polymerase chain reaction. NO and cGMP levels were measured via specific ELISAs. Acetylcholine (ACh)‑induced contraction of single gastric muscle cells preincubated with progesterone was measured via scanning micrometry in the presence or absence of the NO synthase inhibitor, Nω‑Nitro‑L‑arginine (L‑NNA), or guanylyl cyclase inhibitor, 1H‑[1,2,4]Oxadiazolo[4,3‑a]quinoxalin‑1‑one (ODQ), and expressed as percent shortening from resting cell length. PR expression was detected in the stomach muscle cells. Progesterone inhibited ACh‑induced gastric muscle cell contraction. Furthermore, progesterone increased NO and cGMP levels in single gastric muscle cells. Most notably, pre‑incubation of muscle cells with either L‑NNA or ODQ abolished the inhibitory action of progesterone on muscle contraction. These present observations suggest that progesterone promotes muscle cell relaxation in the stomach potentially via the NO/cGMP pathway.

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