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Article

Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction

  • Authors:
    • Elżbieta Grześk
    • Magdalena Mackiewicz‑Milewska
    • Hanna Mackiewicz‑Nartowicz
    • Michał Wiciński
    • Igor Burdziński
    • Maryia Korsak
    • Anna Kopczyńska
    • Wojciech Hagner
    • Grzegorz Grześk
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Hematology and Oncology, Faculty of Medicine, Collegium Medicum, Nicolaus Copernicus University, 85‑094 Bydgoszcz, Poland, Department of Rehabilitation, Faculty of Health Sciences, Collegium Medicum, Nicolaus Copernicus University, 85‑094 Bydgoszcz, Poland, Department of Phoniatry and Audiology, Faculty of Health Sciences, Collegium Medicum, Nicolaus Copernicus University, 85‑094 Bydgoszcz, Poland, Department of Pharmacology and Therapeutics, Faculty of Medicine, Collegium Medicum, Nicolaus Copernicus University, 85‑094 Bydgoszcz, Poland, Second Department of Cardiology, Division of Clinical Pharmacology, Faculty of Health Sciences, Collegium Medicum, Nicolaus Copernicus University, 85‑094 Bydgoszcz, Poland
  • Pages: 23-29
    |
    Published online on: November 11, 2019
       https://doi.org/10.3892/br.2019.1255
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Abstract

Mastoparan‑7 activates guanine nucleotide‑binding proteins (G‑proteins) and stimulates both apoptosis and increases in cytoplasmic calcium concentration and may induce smooth muscle contraction. The primary aim of the present study was to evaluate the modulatory effect of laser stimulation on vascular smooth muscle contraction induced by direct stimulation of G‑protein with mastoparan‑7. Experiments were performed on isolated and perfused tail arteries of Wistar rats. Contraction force in the model was measured by increased levels of perfusion pressure with a constant flow. Irradiation treatment was applied directly to the blood vessels. The laser was applied in increasing doses of 10 mW (E=1.8 J), 30 mW (E=5.5 J) and 110 mW (E=19.8 J). Time of exposure was 3 min for each irradiation. In the laser‑stimulated arteries, a significant and dose‑dependent decrease was observed. The half maximal effective concentration values were 4.43±2.2x10‑8, 2.4±0.56x10‑7, 3.2±0.72x10‑7 and 7.7±0.3x10‑7 M/l in the control and 10, 30 and 110 mW laser irradiation groups, respectively. Significant (P<0.001) changes were identified for all laser treatment groups in comparison with the control. When analyzing the function of calcium ion (Ca2+) stores was analyzed, a significant inhibition of influx from both intra‑ and extracellular Ca2+ stores was observed. The results from the present study suggested that contraction induced by direct activation of G‑protein with mastoparan‑7 may by effectively inhibited by laser radiation, and that the effect was associated with an inhibition of Ca2+ influx from both intracellular and extracellular Ca2+ stores.
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Copy and paste a formatted citation
Spandidos Publications style
Grześk E, Mackiewicz‑Milewska M, Mackiewicz‑Nartowicz H, Wiciński M, Burdziński I, Korsak M, Kopczyńska A, Hagner W and Grześk G: Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction. Biomed Rep 12: 23-29, 2020.
APA
Grześk, E., Mackiewicz‑Milewska, M., Mackiewicz‑Nartowicz, H., Wiciński, M., Burdziński, I., Korsak, M. ... Grześk, G. (2020). Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction. Biomedical Reports, 12, 23-29. https://doi.org/10.3892/br.2019.1255
MLA
Grześk, E., Mackiewicz‑Milewska, M., Mackiewicz‑Nartowicz, H., Wiciński, M., Burdziński, I., Korsak, M., Kopczyńska, A., Hagner, W., Grześk, G."Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction". Biomedical Reports 12.1 (2020): 23-29.
Chicago
Grześk, E., Mackiewicz‑Milewska, M., Mackiewicz‑Nartowicz, H., Wiciński, M., Burdziński, I., Korsak, M., Kopczyńska, A., Hagner, W., Grześk, G."Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction". Biomedical Reports 12, no. 1 (2020): 23-29. https://doi.org/10.3892/br.2019.1255
Copy and paste a formatted citation
x
Spandidos Publications style
Grześk E, Mackiewicz‑Milewska M, Mackiewicz‑Nartowicz H, Wiciński M, Burdziński I, Korsak M, Kopczyńska A, Hagner W and Grześk G: Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction. Biomed Rep 12: 23-29, 2020.
APA
Grześk, E., Mackiewicz‑Milewska, M., Mackiewicz‑Nartowicz, H., Wiciński, M., Burdziński, I., Korsak, M. ... Grześk, G. (2020). Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction. Biomedical Reports, 12, 23-29. https://doi.org/10.3892/br.2019.1255
MLA
Grześk, E., Mackiewicz‑Milewska, M., Mackiewicz‑Nartowicz, H., Wiciński, M., Burdziński, I., Korsak, M., Kopczyńska, A., Hagner, W., Grześk, G."Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction". Biomedical Reports 12.1 (2020): 23-29.
Chicago
Grześk, E., Mackiewicz‑Milewska, M., Mackiewicz‑Nartowicz, H., Wiciński, M., Burdziński, I., Korsak, M., Kopczyńska, A., Hagner, W., Grześk, G."Modulatory effect of laser irradiation on mastoparan‑7‑induced contraction". Biomedical Reports 12, no. 1 (2020): 23-29. https://doi.org/10.3892/br.2019.1255
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