Sirolimus and MMF are insufficient immunosuppressants for regulation of the proliferation of CD133+EpCAM+ cell populations in HCC cell lines

  • Authors:
    • Hwajung Kim
    • Kwang-Woong Lee
    • Seung Cheol Oh
    • Min-Young Park
    • Sooin Seo
    • Xue-Li Jin
    • Suk Kyun Hong
    • Kyung Chul Yoon
    • Nam-Joon Yi
    • Kyung-Suk Suh
  • View Affiliations

  • Published online on: October 20, 2020     https://doi.org/10.3892/br.2020.1376
  • Article Number: 69
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Abstract

Studies on effective immunosuppressive strategies for the management of patients undergoing a liver transplantation (LT) due to hepatocellular carcinoma (HCC) are limited. In the present study, immunosuppressive candidates predicted to exhibit beneficial immunosuppressive and tumor‑suppressive effects in patients with HCC were assessed using Huh7 and HEP3B HCC cells, which have high proportions of CD133+EpCAM+ cancer stem cell (CSC) populations. The immunosuppressants assessed were sirolimus, tacrolimus, cyclosporine A and mycophenolate mofetil (MMF), and their activities were assessed on CSCs. Sirolimus and MMF reduced the proliferation of Huh7 and HEP3B cells; however, the proportion of CD133+EpCAM+ was notably increased in treated Huh7 cells. Sirolimus treatment alone resulted in G0‑G1 cell cycle arrest at all doses in all Huh7 and CD133‑EpCAM‑ populations; however, CD133+EpCAM+ populations showed only slight G1 arrest at higher doses only. In contrast, S‑phase arrest was induced at all doses in the Huh7, CD133‑EpCAM‑ and CD133+EpCAM+ populations by MMF. Sirolimus and MMF effectively reduced the proliferation of Huh7 and HEP3B cells, but did not exert a notable effect on the CD133+EpCAM+ cells. Therefore, therapeutic strategies utilizing Sirolimus and MMF should be further studied in vivo for regulation of CSC populations in order to reduce HCC recurrence rates.
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December-2020
Volume 13 Issue 6

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Kim H, Lee K, Oh SC, Park M, Seo S, Jin X, Hong SK, Yoon KC, Yi N, Suh K, Suh K, et al: Sirolimus and MMF are insufficient immunosuppressants for regulation of the proliferation of CD133+EpCAM+ cell populations in HCC cell lines. Biomed Rep 13: 69, 2020.
APA
Kim, H., Lee, K., Oh, S.C., Park, M., Seo, S., Jin, X. ... Suh, K. (2020). Sirolimus and MMF are insufficient immunosuppressants for regulation of the proliferation of CD133+EpCAM+ cell populations in HCC cell lines. Biomedical Reports, 13, 69. https://doi.org/10.3892/br.2020.1376
MLA
Kim, H., Lee, K., Oh, S. C., Park, M., Seo, S., Jin, X., Hong, S. K., Yoon, K. C., Yi, N., Suh, K."Sirolimus and MMF are insufficient immunosuppressants for regulation of the proliferation of CD133+EpCAM+ cell populations in HCC cell lines". Biomedical Reports 13.6 (2020): 69.
Chicago
Kim, H., Lee, K., Oh, S. C., Park, M., Seo, S., Jin, X., Hong, S. K., Yoon, K. C., Yi, N., Suh, K."Sirolimus and MMF are insufficient immunosuppressants for regulation of the proliferation of CD133+EpCAM+ cell populations in HCC cell lines". Biomedical Reports 13, no. 6 (2020): 69. https://doi.org/10.3892/br.2020.1376