Open Access

miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells

  • Authors:
    • Yanrong Guo
    • Xiaoxiao Ding
    • Changling Dai
    • Wenwen Wang
    • Jianlin Chen
    • Sai Chen
    • Linjun Yang
    • Guang Chen
  • View Affiliations

  • Published online on: October 4, 2024     https://doi.org/10.3892/br.2024.1873
  • Article Number: 185
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Multiple myeloma (MM) is the second most common type of hematological malignancy globally. Despite application of several new drugs, such as daratumumab, bortezomib/lenalidomide/dexamethasone, in combination with hematopoietic stem cell transplantation, overall prognosis remains poor and the pathological mechanism of MM is still unknown. The present study used TargetScan to predict autophagy‑related 7 (ATG7) as a candidate target gene of microRNA (miR)‑1343‑3p and confirmed the interaction between miR‑1343‑3p and the ATG7 3' untranslated region (3'UTR) using a dual‑luciferase reporter assay. In U266 and RPMI‑8226 MM cell lines, miR‑1343‑3p mimic transfection decreased mRNA and protein levels of ATG7, while miR‑1343‑3p inhibition increased ATG7 expression levels using reverse transcription‑qPCR and western blot analysis. miR‑1343‑3p mimic transfection inhibited U266 and RPMI‑8226 cell survival. Finally, miR‑1343‑3p regulated ATG7 and autophagy in MM cells using western blot analysis. The present findings suggested that miR‑1343‑3p may regulate ATG7 and autophagy by directly targeting the 3'UTR of ATG7. To the best of our knowledge, there are no direct data showing the roles of miR‑1343‑3p in development of MM; however, miR‑1343‑3p may be considered a potential target for MM treatment.
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December-2024
Volume 21 Issue 6

Print ISSN: 2049-9434
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Copy and paste a formatted citation
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Spandidos Publications style
Guo Y, Ding X, Dai C, Wang W, Chen J, Chen S, Yang L and Chen G: miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells. Biomed Rep 21: 185, 2024.
APA
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S. ... Chen, G. (2024). miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells. Biomedical Reports, 21, 185. https://doi.org/10.3892/br.2024.1873
MLA
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S., Yang, L., Chen, G."miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells". Biomedical Reports 21.6 (2024): 185.
Chicago
Guo, Y., Ding, X., Dai, C., Wang, W., Chen, J., Chen, S., Yang, L., Chen, G."miR‑1343‑3p inhibits autophagy by directly targeting ATG7 in multiple myeloma cells". Biomedical Reports 21, no. 6 (2024): 185. https://doi.org/10.3892/br.2024.1873