Efficacy of neutral electrolyzed water vs. common topical antiseptics in the healing of full‑thickness burn: Preclinical trial in a mouse model

Delgado‑Enciso,Ivan; Aurelien‑Cabezas,Nomely S.; Meza‑Robles,Carmen; Walle‑Guillen,Mireya; Hernandez‑Fuentes,Gustavo A.; Cabrera‑Licona,Ariana; Hernandez‑Rangel,Alejandra E.; Delgado‑Machuca,Marina; Rodriguez‑Hernandez,Alejandrina; Beas‑Guzman,Oscar F.; Cardenas‑Aguilar,Citlaly B.; Rodriguez‑Sanchez,Iram P.; Martinez‑Fierro,Margarita L.; Chaviano‑Conesa,Daniel; Paz‑Michel,Brenda A.

doi:10.3892/br.2024.1877

Efficacy of neutral electrolyzed water vs. common topical antiseptics in the healing of full‑thickness burn: Preclinical trial in a mouse model

Authors:
- Ivan Delgado‑Enciso
- Nomely S. Aurelien‑Cabezas
- Carmen Meza‑Robles
- Mireya Walle‑Guillen
- Gustavo A. Hernandez‑Fuentes
- Ariana Cabrera‑Licona
- Alejandra E. Hernandez‑Rangel
- Marina Delgado‑Machuca
- Alejandrina Rodriguez‑Hernandez
- Oscar F. Beas‑Guzman
- Citlaly B. Cardenas‑Aguilar
- Iram P. Rodriguez‑Sanchez
- Margarita L. Martinez‑Fierro
- Daniel Chaviano‑Conesa
- Brenda A. Paz‑Michel
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Affiliations: Department of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, Mexico, Department of Research, State Cancerology Institute of Colima, Health Services of The Mexican Social Security Institute for Welfare (IMSS‑BIENESTAR Colima), Colima 28085, Mexico, Department of Research, Esteripharma SA de CV, Atlacomulco 50450, Mexico, Molecular and Structural Physiology Laboratory, School of Biological Sciences, Autonomous University of Nuevo León, San Nicolás de los Garza 66455, Mexico, Molecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, México
Published online on: October 10, 2024 https://doi.org/10.3892/br.2024.1877
Article Number: 189
Copyright: © Delgado‑Enciso et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Burn injuries impose challenges such as infection risk, pain management, fluid loss, electrolyte imbalance and psychological and emotional impact, on healthcare professionals, requiring effective treatments to enhance wound healing. The present study evaluated the efficacy superoxidized electrolyzed solution (SES), with low (SES‑low) or high (SES‑high) concentrations of active species, alone or in combination with a formulation in gel (G), in comparison with commonly prescribed treatments for burn injury, including nitrofurazone (NF) and silver sulfadiazine (S); normal saline was used as placebo (PI). A scald burn model was established in BALB/c mice. Measurements of the burned area and histological parameters such as inflammatory infiltration state, epithelial regeneration and collagen fibers were evaluated on days 3, 6, 9, 18 and 32 to assess healing score and status. All treatments achieved wound closure at day 32; histopathological parameters indicated that SES‑low and SES‑low + G performed better than the Pl and S groups (P<0.05). All treatments showed a lower count of inflammatory cells compared with S (P<0.05); for collagen deposition and orientation, SES‑low + G showed a more uniform horizontal orientation compared with Pl, SES‑high + G, NF and S groups (P<0.05). SES‑Low was the most effective substance to induce favorable and organized healing, while S was the worst, inducing disorganized closure of the wound due to a pro‑inflammatory effect.

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