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Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride

  • Authors:
    • Kosho Kinoshita
    • Mohammad Said Ashenagar
    • Masaki Tabuchi
    • Hideaki Higashino
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Kinki University School of Medicine, Osaka 589-8511, Japan
  • Pages: 201-212
    |
    Published online on: January 14, 2011
       https://doi.org/10.3892/etm.2011.193
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Abstract

Clarification of the genetic nature and more effective care for hypertension are required, given the high incidences of cardiovascular and cerebrovascular mortality. Thus, we surveyed candidate genes for hypertension with rat whole gene DNA microarrays using three novel methods. Gene expression analyses were conducted as follows: Method 1, three types of spontaneously hypertensive rat (SHR) substrains, SHR, stroke-prone SHR (SHRSP) and malignant type of SHRSP (M-SHRSP) were used and compared to normotensive Wistar Kyoto rats; Method 2, the expressed genes between rats of different ages were compared for different blood pressures; and Method 3, genes that were expressed in rats treated with or without an acute hypotensive stimulus, the antihypertensive hydralazine hydrochloride, were compared. This approach identified dozens of genes, including Dusp15, Cyp8b1, Armc 3, Gtpbp4, Mettl2, Mapk14, Prkar2b, frame 12, Anxa13, Ephx2, Myr8 and Pcdh9 by Method 1; Cyp2C and Atp12a by Method 2; and Kcnc3, Vnn1, TC560558 and Gabrq and a number of unknown genes by Methods 2 and 3, as probable candidate genes for hypertension in SHR substrains. Ephx2 was previously reported as a candidate gene in SHRs; however other genes were identified for the first time in this study. Since it was not always possible to completely demonstrate that these genes are responsible for hypertension in SHRs, further research into true candidate genes that participate in the genesis of hypertension in SHR substrains is warranted.
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Copy and paste a formatted citation
Spandidos Publications style
Kinoshita K, Ashenagar MS, Tabuchi M and Higashino H: Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride. Exp Ther Med 2: 201-212, 2011.
APA
Kinoshita, K., Ashenagar, M.S., Tabuchi, M., & Higashino, H. (2011). Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride. Experimental and Therapeutic Medicine, 2, 201-212. https://doi.org/10.3892/etm.2011.193
MLA
Kinoshita, K., Ashenagar, M. S., Tabuchi, M., Higashino, H."Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride". Experimental and Therapeutic Medicine 2.2 (2011): 201-212.
Chicago
Kinoshita, K., Ashenagar, M. S., Tabuchi, M., Higashino, H."Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride". Experimental and Therapeutic Medicine 2, no. 2 (2011): 201-212. https://doi.org/10.3892/etm.2011.193
Copy and paste a formatted citation
x
Spandidos Publications style
Kinoshita K, Ashenagar MS, Tabuchi M and Higashino H: Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride. Exp Ther Med 2: 201-212, 2011.
APA
Kinoshita, K., Ashenagar, M.S., Tabuchi, M., & Higashino, H. (2011). Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride. Experimental and Therapeutic Medicine, 2, 201-212. https://doi.org/10.3892/etm.2011.193
MLA
Kinoshita, K., Ashenagar, M. S., Tabuchi, M., Higashino, H."Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride". Experimental and Therapeutic Medicine 2.2 (2011): 201-212.
Chicago
Kinoshita, K., Ashenagar, M. S., Tabuchi, M., Higashino, H."Whole rat DNA array survey for candidate genes related to hypertension in kidneys from three spontaneously hypertensive rat substrains at two stages of age and with hypotensive induction caused by hydralazine hydrochloride". Experimental and Therapeutic Medicine 2, no. 2 (2011): 201-212. https://doi.org/10.3892/etm.2011.193
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