Effects of peripherally administered urocortin 3 on feeding behavior and gastric emptying in mice

Terashi,Mutsumi; Asakawa,Akihiro; Cheng,Kai-Chun; Koyama,Ken-Ichiro; Chaolu,Huke; Ushikai,Miharu; Inui,Akio

doi:10.3892/etm.2011.200

Effects of peripherally administered urocortin 3 on feeding behavior and gastric emptying in mice

Authors:
- Mutsumi Terashi
- Akihiro Asakawa
- Kai-Chun Cheng
- Ken-Ichiro Koyama
- Huke Chaolu
- Miharu Ushikai
- Akio Inui
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Affiliations: Department of Psychosomatic Internal Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-0852, Japan
Published online on: January 20, 2011 https://doi.org/10.3892/etm.2011.200
Pages: 333-335

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Abstract

Human and mouse urocortin 3 (Ucn3) were first identified in 2001. Ucn3 binds selectively to corticotropin-releasing factor receptor type 2 (CRF-R2). Previous studies have shown that centrally administered Ucn3 decreases food intake in rats. However, the role of Ucn3 in the regulation of gut motility remains to be determined. In the present study, we investigated the effects of peripherally administered Ucn3 on food intake and gastric emptying in mice. After intraperitoneal (i.p.) administration of Ucn3, food intake was measured in the light and dark phases, and the rate of gastric emptying was determined. We found that i.p. administration of Ucn3 significantly inhibited feeding behavior in mice, and significantly delayed gastric emptying 1-2 h after administration in a dose-dependent manner. These results suggest that Ucn3 contributes to the modulation of feeding behavior and gut motility. Thus, Ucn3 and CRF-R2 may be involved in the pathogenesis of functional gastrointestinal and eating disorders.

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1.	Vale W, Spiess J, Rivier C and Rivier J: Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin. Science. 213:1394–1397. 1981. View Article : Google Scholar : PubMed/NCBI
2.	Spiess J, Rivier J, Rivier C and Vale W: Primary structure of corticotropin-releasing factor from ovine hypothalamus. Proc Natl Acad Sci USA. 78:6517–6521. 1981. View Article : Google Scholar : PubMed/NCBI
3.	Denver RJ: Structural and functional evolution of vertebrate neuroendocrine stress systems. Ann NY Acad Sci. 1163:1–16. 2009. View Article : Google Scholar : PubMed/NCBI
4.	Perrin MH, Donaldson CJ, Chen R, Lewis KA and Vale WW: Cloning and functional expression of a rat brain corticotropin releasing factor (CRF) receptor. Endocrinology. 133:3058–3061. 1993.PubMed/NCBI
5.	Chen R, Lewis KA, Perrin MH and Vale WW: Expression cloning of a human corticotropin-releasing-factor receptor. Proc Natl Acad Sci USA. 90:8967–8971. 1993. View Article : Google Scholar : PubMed/NCBI
6.	Vita N, Laurent P, Lefort S, et al: Primary structure and functional expression of mouse pituitary and human brain corticotrophin releasing factor receptors. FEBS Lett. 335:1–5. 1993. View Article : Google Scholar : PubMed/NCBI
7.	Chang CP, Pearse RV II, O'Connell S and Rosenfeld MG: Identification of a seven transmembrane helix receptor for corticotropin-releasing factor and sauvagine in mammalian brain. Neuron. 11:1187–1195. 1993. View Article : Google Scholar : PubMed/NCBI
8.	Potter E, Sutton S, Donaldson C, et al: Distribution of corticotropin-releasing factor receptor mRNA expression in the rat brain and pituitary. Proc Natl Acad Sci USA. 91:8777–8781. 1994. View Article : Google Scholar : PubMed/NCBI
9.	Lovenberg TW, Liaw CW, Grigoriadis DE, et al: Cloning and characterization of a functionally distinct corticotropin-releasing factor receptor subtype from rat brain. Proc Natl Acad Sci USA. 92:836–840. 1995. View Article : Google Scholar : PubMed/NCBI
10.	Chalmers DT, Lovenberg TW and De Souza EB: Localization of novel corticotropin-releasing factor receptor (CRF2) mRNA expression to specific subcortical nuclei in rat brain: comparison with CRF1 receptor mRNA expression. J Neurosci. 15:6340–6350. 1995.PubMed/NCBI
11.	Vaughan J, Donaldson C, Bittencourt J, et al: Urocortin, a mammalian neuropeptide related to fish urotensin I and to corticotropin-releasing factor. Nature. 378:287–292. 1995. View Article : Google Scholar : PubMed/NCBI
12.	Reyes TM, Lewis K, Perrin MH, et al: Urocortin II: a member of the corticotropin-releasing factor (CRF) neuropeptide family that is selectively bound by type 2 CRF receptors. Proc Natl Acad Sci USA. 98:2843–2848. 2001. View Article : Google Scholar : PubMed/NCBI
13.	Hsu SY and Hsueh AJ: Human stresscopin and stresscopin-related peptide are selective ligands for the type 2 corticotropin-releasing hormone receptor. Nat Med. 7:605–611. 2001. View Article : Google Scholar : PubMed/NCBI
14.	Lewis K, Li C, Perrin MH, et al: Identification of urocortin III, an additional member of the corticotropin-releasing factor (CRF) family with high affinity for the CRF2 receptor. Proc Natl Acad Sci USA. 98:7570–7575. 2001. View Article : Google Scholar : PubMed/NCBI
15.	Ohata H and Shibasaki T: Effects of urocortin 2 and 3 on motor activity and food intake in rats. Peptides. 25:1703–1709. 2004. View Article : Google Scholar : PubMed/NCBI
16.	Czimmer J, Million M and Taché Y: Urocortin 2 acts centrally to delay gastric emptying through sympathetic pathways while CRF and urocortin 1 inhibitory actions are vagal dependent in rats. Am J Physiol Gastrointest Liver Physiol. 290:G511–G518. 2006. View Article : Google Scholar : PubMed/NCBI
17.	Tanaka C, Asakawa A, Ushikai M, et al: Comparison of the anorexigenic activity of CRF family peptides. Biochem Biophys Res Commun. 390:887–891. 2009. View Article : Google Scholar : PubMed/NCBI
18.	Duggan JP and Booth DA: Obesity, overeating, and rapid gastric emptying in rats with ventromedial hypothalamic lesions. Science. 231:609–611. 1986. View Article : Google Scholar : PubMed/NCBI
19.	Phillips RJ and Powley TL: Gastric volume rather than nutrient content inhibits food intake. Am J Physiol. 271:R766–R769. 1996.PubMed/NCBI
20.	Gourcerol G, Wang L, Wang YH, Million M and Taché Y: Urocortins and cholecystokinin-8 act synergistically to increase satiation in lean but not obese mice: involvement of corticotropin-releasing factor receptor-2 pathway. Endocrinology. 148:6115–6123. 2007. View Article : Google Scholar : PubMed/NCBI
21.	Liu S, Ren W, Qu MH, et al: Differential actions of urocortins on neurons of the myenteric division of the enteric nervous system in guinea pig distal colon. Br J Pharmacol. 159:222–236. 2010. View Article : Google Scholar : PubMed/NCBI
22.	Chatzaki E, Murphy BJ, Wang L, et al: Differential profile of CRF receptor distribution in the rat stomach and duodenum assessed by newly developed CRF receptor antibodies. J Neurochem. 88:1–11. 2004. View Article : Google Scholar : PubMed/NCBI