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Article

Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

  • Authors:
    • Hyun-Jin Lee
    • Chul-Hong Park
    • Hyung-U Son
    • Jin-Chul Heo
    • Sung-Hee Nam
    • Kwang-Gil Lee
    • Joo-Hong Yeo
    • Cheol-Sik Yoon
    • Jong-Myeung Kim
    • Yong-Kyu Shin
    • Si-Oh Kim
    • Sang-Han Lee
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea, Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Republic of Korea, Department of Agricutural Biology, National Advanced Institute of Science and Technology, RDA, Suwon 441-707, Republic of Korea, Mycoplus Co., Ltd., Uiwang 437-820, Republic of Korea, Bion Co., Ltd., Daegu 702-701, Republic of Korea, Department of Anesthesiology, Kyungpook National University School of Medicine, Daegu 700-412, Republic of Korea
  • Pages: 357-362
    |
    Published online on: January 20, 2011
       https://doi.org/10.3892/etm.2011.214
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Abstract

The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lee H, Park C, Son H, Heo J, Nam S, Lee K, Yeo J, Yoon C, Kim J, Shin Y, Shin Y, et al: Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3. Exp Ther Med 2: 357-362, 2011.
APA
Lee, H., Park, C., Son, H., Heo, J., Nam, S., Lee, K. ... Lee, S. (2011). Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3. Experimental and Therapeutic Medicine, 2, 357-362. https://doi.org/10.3892/etm.2011.214
MLA
Lee, H., Park, C., Son, H., Heo, J., Nam, S., Lee, K., Yeo, J., Yoon, C., Kim, J., Shin, Y., Kim, S., Lee, S."Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3". Experimental and Therapeutic Medicine 2.2 (2011): 357-362.
Chicago
Lee, H., Park, C., Son, H., Heo, J., Nam, S., Lee, K., Yeo, J., Yoon, C., Kim, J., Shin, Y., Kim, S., Lee, S."Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3". Experimental and Therapeutic Medicine 2, no. 2 (2011): 357-362. https://doi.org/10.3892/etm.2011.214
Copy and paste a formatted citation
x
Spandidos Publications style
Lee H, Park C, Son H, Heo J, Nam S, Lee K, Yeo J, Yoon C, Kim J, Shin Y, Shin Y, et al: Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3. Exp Ther Med 2: 357-362, 2011.
APA
Lee, H., Park, C., Son, H., Heo, J., Nam, S., Lee, K. ... Lee, S. (2011). Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3. Experimental and Therapeutic Medicine, 2, 357-362. https://doi.org/10.3892/etm.2011.214
MLA
Lee, H., Park, C., Son, H., Heo, J., Nam, S., Lee, K., Yeo, J., Yoon, C., Kim, J., Shin, Y., Kim, S., Lee, S."Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3". Experimental and Therapeutic Medicine 2.2 (2011): 357-362.
Chicago
Lee, H., Park, C., Son, H., Heo, J., Nam, S., Lee, K., Yeo, J., Yoon, C., Kim, J., Shin, Y., Kim, S., Lee, S."Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3". Experimental and Therapeutic Medicine 2, no. 2 (2011): 357-362. https://doi.org/10.3892/etm.2011.214
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