Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Xian,Yan-Fang; Li,Yu-Cui; Ip,Siu-Po; Lin,Zhi-Xiu; Lai,Xiao-Ping; Su,Zi-Ren

doi:10.3892/etm.2011.233

Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Authors:
- Yan-Fang Xian
- Yu-Cui Li
- Siu-Po Ip
- Zhi-Xiu Lin
- Xiao-Ping Lai
- Zi-Ren Su
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Affiliations: School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China, College of Chinese Medicines, Guangzhou University of Chinese Medicine, Guangzhou, P.R. China
Published online on: March 21, 2011 https://doi.org/10.3892/etm.2011.233
Pages: 545-550

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Abstract

Pogostemonis Herba has long been used in traditional Chinese medicine for the treatment of inflammation-related disorders. Patchouli alcohol (PA) isolated from Pogostemonis Herba is a tricyclic sesquiterpene that is known to exert a variety of pharmacological activities. The present study aimed to investigate the anti-inflammatory effect of PA on lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Pre-treatment with PA at concentrations of 10, 20 or 40 µM dose-dependently decreased the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, nitric oxide (NO) and prostaglandin E2 in LPS-stimulated RAW264.7 cells. In addition, PA treatment also reversed the increased mRNA expression of TNF-α, IL-1β, IL-6, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 caused by LPS in RAW264.7 cells. These results indicate that PA is an important anti-inflammatory constituent of Pogostemonis Herba and that its anti-inflammatory effect may be mediated, at least in part, by down-regulation of the mRNA expression of a panel of inflammatory mediators, such as TNF-α, IL-1β, IL-6, iNOS and COX-2.

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