Bacterial diversity of subgingival plaque in 6 healthy Chinese individuals

Zhang,Song-Mei; Tian,Fei; Huang,Qing-Feng; Zhao,Yan-Fang; Guo,Xiao-Kui; Zhang,Fu-Qiang

doi:10.3892/etm.2011.311

Bacterial diversity of subgingival plaque in 6 healthy Chinese individuals

Authors:
- Song-Mei Zhang
- Fei Tian
- Qing-Feng Huang
- Yan-Fang Zhao
- Xiao-Kui Guo
- Fu-Qiang Zhang
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Affiliations: Department of Prosthodontics, School of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China, Department of Microbiology and Parasitology, Shanghai Jiao Tong University, School of Medicine, Shanghai 200025, P.R. China
Published online on: June 30, 2011 https://doi.org/10.3892/etm.2011.311
Pages: 1023-1029

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Abstract

The subgingival microbial ecology is complex, and little is known regarding its bacteria species composition in healthy Chinese individuals. This study aimed to identify the subgingival microbiota from 6 healthy Chinese subjects. Subgingival samples from 6 volunteers were collected, the 16S rRNA gene was amplified using broad-range bacterial primers, and clone libraries were constructed. For the initial 2,439 sequences analyzed, 383 species-level operational taxonomic units (SLOTUs) belonging to seven phyla were identified, estimated as 51% [95% confidence interval (CI) 44-55] of the SLOTUs in this ecosystem. Most (85%) of the bacterial sequences, falling into 228 types of species, corresponded to known and cultivated species. However, 146 (6%) sequences, comprising 104 phylotypes, had <97% similarity to prior database sequences. Ten bacterial genera were conserved among all 6 individuals, comprising 2,000 (82%) of the 2,439 clones analyzed. Ten species were noted in all of the 6 subjects, comprising 1,435 (58.8%) of the 2,439 clones. Streptococcus infantis was the species most frequently cloned. Furthermore, certain species which may participate in the pathogenesis of periodontal disease were present in the 6 subjects. Although the initial subgingival plaque community of each subject was unique in terms of diversity and composition, 10 common key species were found in the 6 Chinese individuals. These ten species of bacteria in the human subgingival plaque in the 6 healthy individuals may be key species which, to some extent, affect periodontal health. Destruction of these key species in subgingival bacteria may break the microbiota balance and may easily lead to over-breeding conditions resulting in pathogenic oral disease.

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1.	Bik EM, Long CD, Armitage GC, et al: Bacterial diversity in the oral cavity of 10 healthy individuals. ISME J. 4:962–974. 2010. View Article : Google Scholar : PubMed/NCBI
2.	Preza D, Olsen I, Willumsen T, Grinde B and Paster BJ: Diversity and site-specificity of the oral microflora in the elderly. Eur J Clin Microbiol Infect Dis. 28:1033–1040. 2009. View Article : Google Scholar : PubMed/NCBI
3.	Colombo AP, Boches SK, Cotton SL, et al: Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol. 80:1421–1432. 2009. View Article : Google Scholar
4.	Corby PM, Lyons-Weiler J, Bretz WA, et al: Microbial risk indicators of early childhood caries. J Clin Microbiol. 43:5753–5759. 2005. View Article : Google Scholar : PubMed/NCBI
5.	Riggio MP, Lennon A, Rolph HJ, et al: Molecular identification of bacteria on the tongue dorsum of subjects with and without halitosis. Oral Dis. 14:251–258. 2008. View Article : Google Scholar : PubMed/NCBI
6.	Aas JA, Paster BJ, Stokes LN, Olsen I and Dewhirst FE: Defining the normal bacterial flora of the oral cavity. J Clin Microbiol. 43:5721–5732. 2005. View Article : Google Scholar : PubMed/NCBI
7.	Aas JA, Griffen AL, Dardis SR, et al: Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol. 46:1407–1417. 2008. View Article : Google Scholar : PubMed/NCBI
8.	Tanner AC, Kent R Jr, Kanasi E, et al: Clinical characteristics and microbiota of progressing slight chronic periodontitis in adults. J Clin Periodontol. 34:917–930. 2007. View Article : Google Scholar : PubMed/NCBI
9.	Sakamoto M, Rocas IN, Siqueira JF Jr and Benno Y: Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol. 21:112–122. 2006. View Article : Google Scholar : PubMed/NCBI
10.	Tanner AC, Paster BJ, Lu SC, et al: Subgingival and tongue microbiota during early periodontitis. J Dent Res. 85:318–323. 2006. View Article : Google Scholar : PubMed/NCBI
11.	Munson MA, Banerjee A, Watson TF and Wade WG: Molecular analysis of the microflora associated with dental caries. J Clin Microbiol. 42:3023–3029. 2004. View Article : Google Scholar : PubMed/NCBI
12.	Sakamoto M, Huang Y, Umeda M, Ishikawa I and Benno Y: Detection of novel oral phylotypes associated with periodontitis. FEMS Microbiol Lett. 217:65–69. 2002. View Article : Google Scholar : PubMed/NCBI
13.	Becker MR, Paster BJ, Leys EJ, et al: Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol. 40:1001–1009. 2002. View Article : Google Scholar : PubMed/NCBI
14.	Kroes I, Lepp PW and Relman DA: Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci USA. 96:14547–14552. 1999. View Article : Google Scholar : PubMed/NCBI
15.	Paster BJ, Boches SK, Galvin JL, et al: Bacterial diversity in human subgingival plaque. J Bacteriol. 183:3770–3783. 2001. View Article : Google Scholar : PubMed/NCBI
16.	Schloss PD and Handelsman J: Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol. 71:1501–1506. 2005. View Article : Google Scholar : PubMed/NCBI
17.	Huber T, Faulkner G and Hugenholtz P: Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Bioinformatics. 20:2317–2319. 2004. View Article : Google Scholar : PubMed/NCBI
18.	Hughes JB, Hellmann JJ, Ricketts TH and Bohannan BJ: Counting the uncountable: statistical approaches to estimating microbial diversity. Appl Environ Microbiol. 67:4399–4406. 2001. View Article : Google Scholar : PubMed/NCBI
19.	Pavoine S, Dufour AB and Chessel D: From dissimilarities among species to dissimilarities among communities: a double principal coordinate analysis. J Theor Biol. 228:523–537. 2004. View Article : Google Scholar : PubMed/NCBI
20.	Gao Z, Tseng CH, Pei Z and Blaser MJ: Molecular analysis of human forearm superficial skin bacterial biota. Proc Natl Acad Sci USA. 104:2927–2932. 2007. View Article : Google Scholar : PubMed/NCBI
21.	Pearce DA, van der Gast CJ, Woodward K and Newsham KK: Significant changes in the bacterioplankton community structure of a maritime Antarctic freshwater lake following nutrient enrichment. Microbiology. 151:3237–3248. 2005. View Article : Google Scholar
22.	Bin-Nun A, Bromiker R, Wilschanski M, et al: Oral probiotics prevent necrotizing enterocolitis in very low birth weight neonates. J Pediatr. 147:192–196. 2005. View Article : Google Scholar : PubMed/NCBI
23.	Mayanagi G, Sato T, Shimauchi H and Takahashi N: Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects. Oral Microbiol Immunol. 19:379–385. 2004. View Article : Google Scholar
24.	Lodhia P, Yaegaki K, Khakbaznejad A, et al: Effect of green tea on volatile sulfur compounds in mouth air. J Nutr Sci Vitaminol. 54:89–94. 2008. View Article : Google Scholar : PubMed/NCBI
25.	Duckworth RM: The science behind caries prevention. Int Dent J. 43:529–539. 1993.PubMed/NCBI